This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Resto-Ruiz, S. I.
Right arrow Articles by Anderson, B. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Resto-Ruiz, S. I.
Right arrow Articles by Anderson, B. E.

 Previous Article  |  Next Article 

Infection and Immunity, October 2000, p. 5970-5978, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Transcriptional Activation of the htrA (High-Temperature Requirement A) Gene from Bartonella henselae

Sandra I. Resto-Ruiz,1 Debra Sweger,1 Raymond H. Widen,1 Nikola Valkov,2 and Burt E. Anderson1,*

Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida,1 and Moffitt Cancer Research Center,2 Tampa, Florida

Received 26 April 2000/Returned for modification 8 June 2000/Accepted 18 June 2000

Bacterial htrA genes are typically activated as part of the periplasmic stress response and are dependent on the extracytoplasmic sigma factor rpoE. A putative promoter region, P1, of the sigma E-type heat-inducible promoters has previously been identified upstream of the htrA gene of Bartonella henselae. Further analysis of the htrA mRNA by primer extension demonstrated that transcription initiates from P1 and a second region downstream of P1. This second promoter region, termed P2, had no sequence identity to sigma E-type heat-inducible promoters. Promoter regions were cloned individually and in tandem into pANT3 upstream of a promoterless version of the green fluorescent protein (GFP) gene (gfpmut3) and transformed into B. henselae by electroporation. The contiguous promoter region containing both P1 and P2 were necessary for the optimal transcriptional activation of the htrA gene. Promoter activity at 37°C was distinctively higher than at 27°C. However, thermal induction at 47°C did not increase expression of gfpmut3. Invasion of human microvascular endothelial cells (HMEC-1) by B. henselae resulted in the formation of well-defined vacuoles containing clusters of bacteria exhibiting marked expression of gfpmut3 transcribed from the P1-P2 region. In addition, a moderate yet significant increase in the ratio of bacterial GFP to DNA was detected for intracellular bacteria compared to extracellular bacteria, indicating upregulation of htrA upon invasion of HMEC-1. The activation of specific genes in the intracellular environment may help us better understand the novel pathogenic mechanisms used by this bacterium.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology MDC10, College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612. Phone: (813) 974-2608. Fax: (813) 974-4151. E-mail: banderso{at}com1.med.usf.edu.

Infection and Immunity, October 2000, p. 5970-5978, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lewis, C., Skovierova, H., Rowley, G., Rezuchova, B., Homerova, D., Stevenson, A., Spencer, J., Farn, J., Kormanec, J., Roberts, M. (2009). Salmonella enterica Serovar Typhimurium HtrA: regulation of expression and role of the chaperone and protease activities during infection. Microbiology 155: 873-881 [Abstract] [Full Text]  
  • Nevesinjac, A. Z., Raivio, T. L. (2005). The Cpx Envelope Stress Response Affects Expression of the Type IV Bundle-Forming Pili of Enteropathogenic Escherichia coli. J. Bacteriol. 187: 672-686 [Abstract] [Full Text]  
  • Dedieu, L., Pages, J.-M., Bolla, J.-M. (2002). Environmental Regulation of Campylobacter jejuni Major Outer Membrane Protein Porin Expression in Escherichia coli Monitored by Using Green Fluorescent Protein. Appl. Environ. Microbiol. 68: 4209-4215 [Abstract] [Full Text]  
  • Resto-Ruiz, S. I., Schmiederer, M., Sweger, D., Newton, C., Klein, T. W., Friedman, H., Anderson, B. E. (2002). Induction of a Potential Paracrine Angiogenic Loop between Human THP-1 Macrophages and Human Microvascular Endothelial Cells during Bartonella henselae Infection. Infect. Immun. 70: 4564-4570 [Abstract] [Full Text]  
  • Schmiederer, M., Arcenas, R., Widen, R., Valkov, N., Anderson, B. (2001). Intracellular Induction of the Bartonella henselae virB Operon by Human Endothelial Cells. Infect. Immun. 69: 6495-6502 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»