Pseudomonas aeruginosa ExoT Is a Rho GTPase-Activating Protein

doi:10.1128/IAI.68.10.6066-6068.2000

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Infection and Immunity, October 2000, p. 6066-6068, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pseudomonas aeruginosa ExoT Is a Rho GTPase-Activating Protein

Rebecca Krall,¹ Gudula Schmidt,² Klaus Aktories,² and Joseph T. Barbieri¹^,^*

Medical College of Wisconsin, Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226,¹ and Institut für Pharmakologie und Toxikologie, Albert-Ludwigs-Universität Freiburg, D-79104 Freiburg, Germany²

Received 25 April 2000/Returned for modification 20 June 2000/Accepted 31 July 2000

Transient intracellular expression of ExoT in CHO cells stimulated cell rounding and actin reorganization. Biochemical studiesshowed that ExoT was a GTPase-activating protein for RhoA, Rac1,and Cdc42. Together, these data show that ExoT interferes withRho signal transduction pathways, which regulate actin organization,exocytosis, cell cycle progression, andphagocytosis.

^* Corresponding author. Mailing address: Medical College of Wisconsin, Microbiology and Molecular Genetics, 8701 Watertown Plk.Rd., Milwaukee, WI 53226. Phone: (414) 456-8412. Fax: (414) 456-6535.E-mail: toxin{at}mcw.edu.

Infection and Immunity, October 2000, p. 6066-6068, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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