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Infection and Immunity, October 2000, p. 6073-6076, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Increased Numbers of ESAT-6- and Purified Protein Derivative-Specific Gamma Interferon-Producing Cells in Subclinical and Active Tuberculosis Infection

Timo Ulrichs,1,2 Peter Anding,1 Steven Porcelli,2 Stefan H. E. Kaufmann,1 and Martin E. Munk1,*

Department of Immunology, Max Planck Institute for Infection Biology, 10117 Berlin, Germany,1 and Department of Microbiology and Immunology, Albert Einstein College of Medicine, New York, New York2

Received 18 April 2000/Returned for modification 12 May 2000/Accepted 16 July 2000

Numbers of gamma interferon (IFN-gamma )-producing cells reactive to ESAT-6 antigen were increased in recent converters to purified protein derivative positivity and in tuberculosis patients but not in unvaccinated or Mycobacterium bovis BCG-vaccinated healthy donors. ESAT-6-reactive IFN-gamma -producing cells in recent converters and tuberculosis patients recognized similar synthetic peptides. Thus, ESAT-6 is a potential candidate for use in detection of early, as well as active, tuberculosis and for control of the disease.

* Corresponding author. Present address: Department of Tuberculosis Immunology, Statens Serum Institute, 5 Artillerivej, 2300 Copenhagen S, Denmark. Phone: 45-3268 8158. Fax: 45-3268 3035. E-mail: MMN{at}SSI.DK.

Infection and Immunity, October 2000, p. 6073-6076, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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