This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mölne, L.
Right arrow Articles by Tarkowski, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mölne, L.
Right arrow Articles by Tarkowski, A.

 Previous Article  |  Next Article 

Infection and Immunity, November 2000, p. 6162-6167, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Neutrophil Leukocytes in Cutaneous Infection Caused by Staphylococcus aureus

Lena Mölne,1,2,* Margareta Verdrengh,2 and Andrzej Tarkowski2

Departments of Dermatology1 and Rheumatology,2 Sahlgrenska University Hospital, Göteborg, Sweden

Received 2 May 2000/Returned for modification 16 June 2000/Accepted 10 August 2000

Despite the high prevalence of cutaneous infections, little is known about the role of host immune responsiveness during Staphylococcus aureus dermatitis. We have recently described a murine model of infectious dermatitis induced by superantigen-producing S. aureus. To assess the role of neutrophils in staphylococcal dermatitis, mice were given granulocyte-depleting monoclonal antibody prior to and on several occasions following intracutaneous inoculation with staphylococci. The granulocyte-depleted mice that had been intradermally inoculated with S. aureus developed crusted ulcerations which tended not to heal, whereas animals injected with control monoclonal antibody displayed only minor and transient skin lesions. The finding of severe ulcerations in neutropenic mice correlated with a significantly higher burden of bacteria in the blood and skin during the early phase of the infection. Importantly, while mice with an intact granulocyte population showed only limited skin infection, bacteremia occurred in the great majority of the neutrophil-depleted animals. As a consequence, the latter individuals exhibited significantly increased levels of the proinflammatory cytokine interleukin-6 and specific antibodies to staphylococcal cell wall components and toxic shock syndrome toxin-1 in the serum. Our data point to a crucial protective role of granulocytes in S. aureus dermatitis.

* Corresponding author. Mailing address: Department of Rheumatology, University of Göteborg, Guldhedsgatan 10A, 413 46 Göteborg, Sweden. Phone: 46-31-3424021. Fax: 46-31-823925. E-mail: lena.molne{at}immuno.gu.se.

Infection and Immunity, November 2000, p. 6162-6167, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • von Kockritz-Blickwede, M., Rohde, M., Oehmcke, S., Miller, L. S., Cheung, A. L., Herwald, H., Foster, S., Medina, E. (2008). Immunological Mechanisms Underlying the Genetic Predisposition to Severe Staphylococcus aureus Infection in the Mouse Model. Am. J. Pathol. 173: 1657-1668 [Abstract] [Full Text]  
  • Fujisawa, H. (2008). Neutrophils Play an Essential Role in Cooperation with Antibody in both Protection against and Recovery from Pulmonary Infection with Influenza Virus in Mice. J. Virol. 82: 2772-2783 [Abstract] [Full Text]  
  • Miller, L. S., Pietras, E. M., Uricchio, L. H., Hirano, K., Rao, S., Lin, H., O'Connell, R. M., Iwakura, Y., Cheung, A. L., Cheng, G., Modlin, R. L. (2007). Inflammasome-Mediated Production of IL-1beta Is Required for Neutrophil Recruitment against Staphylococcus aureus In Vivo. J. Immunol. 179: 6933-6942 [Abstract] [Full Text]  
  • Hunt, C. L., Nauseef, W. M., Weiss, J. P. (2006). Effect of D-Alanylation of (Lipo)Teichoic Acids of Staphylococcus aureus on Host Secretory Phospholipase A2 Action before and after Phagocytosis by Human Neutrophils.. J. Immunol. 176: 4987-4994 [Abstract] [Full Text]  
  • Voyich, J. M., Braughton, K. R., Sturdevant, D. E., Whitney, A. R., Said-Salim, B., Porcella, S. F., Long, R. D., Dorward, D. W., Gardner, D. J., Kreiswirth, B. N., Musser, J. M., DeLeo, F. R. (2005). Insights into Mechanisms Used by Staphylococcus aureus to Avoid Destruction by Human Neutrophils. J. Immunol. 175: 3907-3919 [Abstract] [Full Text]  
  • Wright, J. S. III, Jin, R., Novick, R. P. (2005). Transient interference with staphylococcal quorum sensing blocks abscess formation. Proc. Natl. Acad. Sci. USA 102: 1691-1696 [Abstract] [Full Text]  
  • Shi, L., Takahashi, K., Dundee, J., Shahroor-Karni, S., Thiel, S., Jensenius, J. C., Gad, F., Hamblin, M. R., Sastry, K. N., Ezekowitz, R. A. B. (2004). Mannose-binding Lectin-deficient Mice Are Susceptible to Infection with Staphylococcus aureus. JEM 199: 1379-1390 [Abstract] [Full Text]  
  • Ahmed, H. J., Johansson, C., Svensson, L. A., Ahlman, K., Verdrengh, M., Lagergard, T. (2002). In Vitro and In Vivo Interactions of Haemophilusducreyi with Host Phagocytes. Infect. Immun. 70: 899-908 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»