This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morikawa, A.
Right arrow Articles by Yokochi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morikawa, A.
Right arrow Articles by Yokochi, T.

 Previous Article  |  Next Article 

Infection and Immunity, November 2000, p. 6209-6214, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Augmentation of Nitric Oxide Production by Gamma Interferon in a Mouse Vascular Endothelial Cell Line and Its Modulation by Tumor Necrosis Factor Alpha and Lipopolysaccharide

Akiko Morikawa, Naoki Koide, Yutaka Kato, Tsuyoshi Sugiyama, Dipshikha Chakravortty, Tomoaki Yoshida, and Takashi Yokochi*

Department of Microbiology and Immunology and Division of Bacterial Toxins, Research Center for Infectious Diseases, Aichi Medical University, Nagakute, Aichi 480-1195, Japan

Received 13 March 2000/Returned for modification 10 July 2000/Accepted 11 August 2000

The effect of gamma interferon (IFN-gamma ), tumor necrosis factor alpha (TNF-alpha ), and lipopolysaccharide (LPS) on nitric oxide (NO) production in the mouse vascular aortic endothelial cell line END-D was examined. LPS, TNF-alpha , and a low concentration of IFN-gamma inhibited NO production in END-D cells, while a high concentration of IFN-gamma definitely enhanced it. The NO production induced by a high concentration of IFN-gamma was further augmented by using IFN-gamma in combination with LPS or TNF-alpha . In sequential incubations of LPS and IFN-gamma , the enhancement of NO production required prior treatment with IFN-gamma . Stimulation of END-D cells with a high concentration of IFN-gamma led to the expression of inducible NO synthase (iNOS). The augmentation of NO production by IFN-gamma alone or in combination with LPS or TNF-alpha was completely blocked by several inhibitors of iNOS. It was strongly suggested that a high concentration of IFN-gamma itself enhanced NO production in END-D cells through inducing the expression of iNOS. LPS and TNF-alpha exclusively modulated the activity of iNOS once its expression was triggered by IFN-gamma . On the other hand, a low concentration of IFN-gamma , LPS, and TNF-alpha reduced NO production through down-regulating constitutive NOS (cNOS). The differential regulation of cNOS- and iNOS-mediated NO production by IFN-gamma , TNF-alpha , and LPS is discussed.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Aichi Medical University, Nagakute, Aichi 480-1195, Japan. Phone: 81-561-62-3311, ext. 2269. Fax: 81-561-63-9187. E-mail: yokochi{at}aichi-med-u.ac.jp.

Infection and Immunity, November 2000, p. 6209-6214, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhou, Z., Gengaro, P., Wang, W., Wang, X.-q., Li, C., Faubel, S., Rivard, C., Schrier, R. W. (2008). Role of NF-{kappa}B and PI 3-kinase/Akt in TNF-{alpha}-induced cytotoxicity in microvascular endothelial cells. Am. J. Physiol. Renal Physiol. 295: F932-F941 [Abstract] [Full Text]  
  • Liu, T., Huang, Y., Likhotvorik, R. I., Keshvara, L., Hoyt, D. G. (2008). Protein Never in Mitosis Gene A Interacting-1 (PIN1) regulates degradation of inducible nitric oxide synthase in endothelial cells. Am. J. Physiol. Cell Physiol. 295: C819-C827 [Abstract] [Full Text]  
  • Koide, N., Mya Mya Mu, , Hassan, F., Islam, S., Tumurkhuu, G., Dagvadorj, J., Naiki, Y., Mori, I., Yoshida, T., Yokochi, T. (2007). Lipopolysaccharide enhances interferon-{gamma}-induced nitric oxide (NO) production in murine vascular endothelial cells via augmentation of interferon regulatory factor-1 activation. Innate Immunity 13: 167-175 [Abstract]  
  • Huang, H., Rose, J. L., Hoyt, D. G. (2004). p38 Mitogen-Activated Protein Kinase Mediates Synergistic Induction of Inducible Nitric-Oxide Synthase by Lipopolysaccharide and Interferon-{gamma} through Signal Transducer and Activator of Transcription 1 Ser727 Phosphorylation in Murine Aortic Endothelial Cells. Mol. Pharmacol. 66: 302-311 [Abstract] [Full Text]  
  • Tai, S. C., Robb, G. B., Marsden, P. A. (2004). Endothelial Nitric Oxide Synthase: A New Paradigm for Gene Regulation in the Injured Blood Vessel. Arterioscler. Thromb. Vasc. Bio. 24: 405-412 [Abstract] [Full Text]  
  • Morikawa, A., Sugiyama, T., Koide, N., Mori, I., Mya Mya Mu, , Yoshida, T., Hassan, F., Islam, S., Yokochi, T. (2004). Butyrate enhances the production of nitric oxide in mouse vascular endothelial cells in response to gamma interferon. Innate Immunity 10: 33-38 [Abstract]  
  • Sugiyama, T., Fujita, M., Koide, N., Morikawa, A., Takahashi, K., Yoshida, T., Mori, H., Yokochi, T. (2003). Differences in the mechanism of nitric oxide production between mouse vascular endothelial cells and macrophages. Innate Immunity 9: 108-112 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»