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Infection and Immunity, November 2000, p. 6281-6288, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Cloning and Analysis of a Putative Siderophore ABC Transporter from Staphylococcus aureus

Julie A. Morrissey,1,* Alan Cockayne,1 Philip J. Hill,1,2 and Paul Williams1,3

Institute of Infections and Immunity,1 Department of Applied Biochemistry and Food Science,2 and School of Pharmaceutical Sciences,3 University of Nottingham, Nottingham NG7 2UH, United Kingdom

Received 26 June 2000/Returned for modification 24 July 2000/Accepted 16 August 2000

From a mass-excised Staphylococcus aureus lambda ZapII expression library, we cloned an operon encoding a novel ABC transporter with significant homology to bacterial siderophore transporter systems. The operon encodes four genes designated sstA, -B, -C, and -D encoding two putative cytoplasmic membrane proteins (sstA and sstB), an ATPase (sstC), and a membrane-bound 38-kDa lipoprotein (sstD). The sst operon is preceded by two putative Fur boxes, which indicated that expression of the sst operon was likely to be iron dependent. SstD was overexpressed in Escherichia coli, purified by Triton X-114 phase partitioning, and used to generate monospecific antisera in rats. Immunoblotting studies located SstD in the membrane fraction of S. aureus and showed that expression of the lipoprotein was reduced under iron-rich growth conditions. Triton X-114 partitioning studies on isolated membranes provided additional biochemical evidence that SstD in S. aureus is a lipoprotein. Immunoreactive polypeptides of approximately 38 kDa were detected in a wide range of staphylococcal species, but no antigenic homolog was detected in Bacillus subtilis. Expression of SstD in vivo was confirmed by immunoblotting studies with S. aureus recovered from a rat intraperitoneal chamber implant model. To further define the contribution of SstD in promoting growth of S. aureus in vitro and in vivo, we used antisense RNA technology to modulate expression of SstD. Expression of antisense sstD RNA in S. aureus resulted in a decrease in SstD expression under both iron-rich and iron-restricted growth conditions. However, this reduction in SstD levels did not affect the growth of S. aureus in vitro in an iron-limited growth medium or when grown in an intraperitoneal rat chamber implant model in vivo.

* Corresponding author. Present address: Department of Microbiology & Immunology, University of Leicester, P.O. Box 138, Medical Sciences Building, University Rd., Leicester LE1 9HN, United Kingdom. Phone: 44-116-2522943. Fax: 44-116-2525030. E-mail: jam26{at}le.ac.uk.

Infection and Immunity, November 2000, p. 6281-6288, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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