This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Day, W. A.
Right arrow Articles by Joens, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Day, W. A., Jr.
Right arrow Articles by Joens, L. A.

 Previous Article  |  Next Article 

Infection and Immunity, November 2000, p. 6337-6345, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Catalase in Campylobacter jejuni Intracellular Survival

William A. Day Jr.,1 Jaime L. Sajecki,2 Todd M. Pitts,2 and Lynn A. Joens2,*

Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721,2 and Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-47991

Received 15 February 2000/Returned for modification 8 May 2000/Accepted 2 August 2000

The ability of Campylobacter jejuni to penetrate normally nonphagocytic host cells is believed to be a key virulence determinant. Recently, kinetics of C. jejuni intracellular survival have been described and indicate that the bacterium can persist and multiply within epithelial cells and macrophages in vitro. Studies conducted by Pesci et al. indicate that superoxide dismutase contributes to intraepithelial cell survival, as isogenic sod mutants are 12-fold more sensitive to intracellular killing than wild-type strains. These findings suggest that bacterial factors that combat reactive oxygen species enable the organism to persist inside host cells. Experiments were conducted to determine the contribution of catalase to C. jejuni intracellular survival. Zymographic analysis indicated that C. jejuni expresses a single catalase enzyme. The gene encoding catalase (katA) was cloned via functional complementation, and an isogenic katA mutant strain was constructed. Kinetic studies indicate that catalase provides resistance to hydrogen peroxide in vitro but does not play a role in intraepithelial cell survival. Catalase does however contribute to intramacrophage survival. Kinetic studies of C. jejuni growth in murine and porcine peritoneal macrophages demonstrated extensive killing of both wild-type and katA mutant strains shortly following internalization. Long-term cultures (72 h postinfection) of infected phagocytes permitted recovery of viable wild-type C. jejuni; in contrast, no viable katA mutant bacteria were recovered. Accordingly, inhibition of macrophage nitric oxide synthase or NADPH oxidase permitted recovery of katA mutant C. jejuni. These observations indicate that catalase is essential for C. jejuni intramacrophage persistence and growth and suggest a novel mechanism of intracellular survival.

* Corresponding author. Mailing address: Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, AZ 85721. Phone: (520) 621-4148. Fax: (520) 621-6366. E-mail: joens{at}ag.arizona.edu.

Infection and Immunity, November 2000, p. 6337-6345, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lin, A. E., Krastel, K., Hobb, R. I., Thompson, S. A., Cvitkovitch, D. G., Gaynor, E. C. (2009). Atypical Roles for Campylobacter jejuni Amino Acid ATP Binding Cassette Transporter Components PaqP and PaqQ in Bacterial Stress Tolerance and Pathogen-Host Cell Dynamics. Infect. Immun. 77: 4912-4924 [Abstract] [Full Text]  
  • Chun, H., Choi, O., Goo, E., Kim, N., Kim, H., Kang, Y., Kim, J., Moon, J. S., Hwang, I. (2009). The Quorum Sensing-Dependent Gene katG of Burkholderia glumae Is Important for Protection from Visible Light. J. Bacteriol. 191: 4152-4157 [Abstract] [Full Text]  
  • Martinez-Pulgarin, S., Dominguez-Bernal, G., Orden, J. A., de la Fuente, R. (2009). Simultaneous lack of catalase and beta-toxin in Staphylococcus aureus leads to increased intracellular survival in macrophages and epithelial cells and to attenuated virulence in murine and ovine models. Microbiology 155: 1505-1515 [Abstract] [Full Text]  
  • Iovine, N. M., Pursnani, S., Voldman, A., Wasserman, G., Blaser, M. J., Weinrauch, Y. (2008). Reactive Nitrogen Species Contribute to Innate Host Defense against Campylobacter jejuni. Infect. Immun. 76: 986-993 [Abstract] [Full Text]  
  • Bingham-Ramos, L. K., Hendrixson, D. R. (2008). Characterization of Two Putative Cytochrome c Peroxidases of Campylobacter jejuni Involved in Promoting Commensal Colonization of Poultry. Infect. Immun. 76: 1105-1114 [Abstract] [Full Text]  
  • Candon, H. L., Allan, B. J., Fraley, C. D., Gaynor, E. C. (2007). Polyphosphate Kinase 1 Is a Pathogenesis Determinant in Campylobacter jejuni. J. Bacteriol. 189: 8099-8108 [Abstract] [Full Text]  
  • Cogan, T A, Thomas, A O, Rees, L E N, Taylor, A H, Jepson, M A, Williams, P H, Ketley, J, Humphrey, T J (2007). Norepinephrine increases the pathogenic potential of Campylobacter jejuni. Gut 56: 1060-1065 [Abstract] [Full Text]  
  • Hong, Y., Wang, G., Maier, R. J. (2007). A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage. J Med Microbiol 56: 557-562 [Abstract] [Full Text]  
  • Naikare, H., Palyada, K., Panciera, R., Marlow, D., Stintzi, A. (2006). Major Role for FeoB in Campylobacter jejuni Ferrous Iron Acquisition, Gut Colonization, and Intracellular Survival.. Infect. Immun. 74: 5433-5444 [Abstract] [Full Text]  
  • Giles, S. S., Stajich, J. E., Nichols, C., Gerrald, Q. D., Alspaugh, J. A., Dietrich, F., Perfect, J. R. (2006). The Cryptococcus neoformans Catalase Gene Family and Its Role in Antioxidant Defense.. Eukaryot Cell 5: 1447-1459 [Abstract] [Full Text]  
  • Palyada, K., Threadgill, D., Stintzi, A. (2004). Iron Acquisition and Regulation in Campylobacter jejuni. J. Bacteriol. 186: 4714-4729 [Abstract] [Full Text]  
  • Carrillo, C. D., Taboada, E., Nash, J. H. E., Lanthier, P., Kelly, J., Lau, P. C., Verhulp, R., Mykytczuk, O., Sy, J., Findlay, W. A., Amoako, K., Gomis, S., Willson, P., Austin, J. W., Potter, A., Babiuk, L., Allan, B., Szymanski, C. M. (2004). Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA. J. Biol. Chem. 279: 20327-20338 [Abstract] [Full Text]  
  • Siegesmund, A. M., Konkel, M. E., Klena, J. D., Mixter, P. F. (2004). Campylobacter jejuni infection of differentiated THP-1 macrophages results in interleukin 1{beta} release and caspase-1-independent apoptosis. Microbiology 150: 561-569 [Abstract] [Full Text]  
  • Srinivasa Rao, P. S., Yamada, Y., Leung, K. Y. (2003). A major catalase (KatB) that is required for resistance to H2O2 and phagocyte-mediated killing in Edwardsiella tarda. Microbiology 149: 2635-2644 [Abstract] [Full Text]  
  • Sabarth, N., Lamer, S., Zimny-Arndt, U., Jungblut, P. R., Meyer, T. F., Bumann, D. (2002). Identification of Surface Proteins of Helicobacter pylori by Selective Biotinylation, Affinity Purification, and Two-dimensional Gel Electrophoresis. J. Biol. Chem. 277: 27896-27902 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»