This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sokol, P. A.
Right arrow Articles by Kooi, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sokol, P. A.
Right arrow Articles by Kooi, C. D.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 6554-6560, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of a Siderophore Receptor Required for Ferric Ornibactin Uptake in Burkholderia cepacia

P. A. Sokol,* P. Darling, S. Lewenza, C. R. Corbett, and C. D. Kooi

Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1

Received 5 May 2000/Returned for modification 11 August 2000/Accepted 15 August 2000

Ornibactins are linear hydroxamate siderophores produced by Burkholderia cepacia with peptide structures similar to that of pyoverdines produced by the fluorescent pseudomonads. The gene encoding the outer membrane receptor (orbA) was identified, sequenced, and demonstrated to have significant homology with hydroxamate receptors produced by other organisms. The orbA precursor was predicted to be a protein with a molecular mass of 81 kDa. An orbA mutant was constructed and demonstrated to be unable to take up 59Fe-ornibactins or to grow in medium supplemented with ornibactins. Outer membrane protein profiles from the parent strain, K56-2, revealed an iron-regulated outer membrane protein of 78 kDa that was not detectable in the K56orbA::tp mutant. When this mutant harbored a plasmid containing the orbA gene, the 78-kDa protein was present in the outer membrane protein profiles and the mutant was able to utilize ornibactin to acquire iron. The orbA mutant was less virulent in a chronic respiratory infection model than the parent strain, indicating that ornibactin uptake and utilization are important in the pathogenesis of B. cepacia respiratory infections.

* Corresponding author. Mailing address: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Dr. N.W., Calgary, Alberta, Canada T2N 4N1. Phone: (403) 220-6037. Fax: (403) 270-2772. E-mail: psokol{at}ucalgary.ca.

Infection and Immunity, December 2000, p. 6554-6560, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Uehlinger, S., Schwager, S., Bernier, S. P., Riedel, K., Nguyen, D. T., Sokol, P. A., Eberl, L. (2009). Identification of Specific and Universal Virulence Factors in Burkholderia cenocepacia Strains by Using Multiple Infection Hosts. Infect. Immun. 77: 4102-4110 [Abstract] [Full Text]  
  • Agnoli, K., Lowe, C. A., Farmer, K. L., Husnain, S. I., Thomas, M. S. (2006). The Ornibactin Biosynthesis and Transport Genes of Burkholderia cenocepacia Are Regulated by an Extracytoplasmic Function {sigma} Factor Which Is a Part of the Fur Regulon.. J. Bacteriol. 188: 3631-3644 [Abstract] [Full Text]  
  • Alice, A. F., Lopez, C. S., Lowe, C. A., Ledesma, M. A., Crosa, J. H. (2006). Genetic and Transcriptional Analysis of the Siderophore Malleobactin Biosynthesis and Transport Genes in the Human Pathogen Burkholderia pseudomallei K96243. J. Bacteriol. 188: 1551-1566 [Abstract] [Full Text]  
  • Visser, M. B., Majumdar, S., Hani, E., Sokol, P. A. (2004). Importance of the Ornibactin and Pyochelin Siderophore Transport Systems in Burkholderia cenocepacia Lung Infections. Infect. Immun. 72: 2850-2857 [Abstract] [Full Text]  
  • Farmer, K. L., Thomas, M. S. (2004). Isolation and Characterization of Burkholderia cenocepacia Mutants Deficient in Pyochelin Production: Pyochelin Biosynthesis Is Sensitive to Sulfur Availability. J. Bacteriol. 186: 270-277 [Abstract] [Full Text]  
  • Sokol, P. A., Sajjan, U., Visser, M. B., Gingues, S., Forstner, J., Kooi, C. (2003). The CepIR quorum-sensing system contributes to the virulence of Burkholderia cenocepacia respiratory infections. Microbiology 149: 3649-3658 [Abstract] [Full Text]  
  • Bernier, S. P., Silo-Suh, L., Woods, D. E., Ohman, D. E., Sokol, P. A. (2003). Comparative Analysis of Plant and Animal Models for Characterization of Burkholderia cepacia Virulence. Infect. Immun. 71: 5306-5313 [Abstract] [Full Text]  
  • Lewenza, S., Sokol, P. A. (2001). Regulation of Ornibactin Biosynthesis and N-Acyl-L-Homoserine Lactone Production by CepR in Burkholderia cepacia. J. Bacteriol. 183: 2212-2218 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»