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Infection and Immunity, December 2000, p. 6574-6579, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Regulation of the Porphyromonas gingivalis fimA (Fimbrillin) Gene

Hua Xie,1,* Whasun O. Chung,2 Yoonsuk Park,2 and Richard J. Lamont2

School of Dentistry, Meharry Medical College, Nashville, Tennessee,1 and Department of Oral Biology, University of Washington, Seattle, Washington2

Received 25 May 2000/Returned for modification 1 August 2000/Accepted 7 September 2000

In common with many bacterial virulence genes, the fimbrillin (fimA) gene of Porphyromonas gingivalis is modulated in response to environmental fluctuation. The trans-acting components that comprise the regulatory system for transcriptional activity of the fimA gene in P. gingivalis were investigated. Three major proteins were found to bind to the upstream region of the fimA promoter. One of these proteins was fimbrillin itself, and the other two were a major arginine protease (Rgp) and lysine protease (Kgp). Production of these proteins was necessary for maximal fimA transcription. An exogenous fimA promoter-lacZ reporter was inactive when introduced into a strain of P. gingivalis carrying a mutation in the indigenous fimA gene. Furthermore, fimA mRNA levels were significantly decreased in rgp and kgp mutant strains. These data indicate that P. gingivalis has evolved multiple levels of control of fimbrial gene expression to enhance its survival in hostile environments.

* Corresponding author. Mailing address: School of Dentistry, Meharry Medical College, Nashville, TN 37208. Phone: (615) 327-5981. Fax: (615) 327-2989. E-mail: hxie{at}mail.mmc.edu.

Infection and Immunity, December 2000, p. 6574-6579, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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