This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Turbyfill, K. R. Articles by Oaks, E. V. Search for Related Content PubMed PubMed Citation Articles by Turbyfill, K. R. Articles by Oaks, E. V.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 6624-6632, Vol. 68, No. 12
0019-9567/00/$04.00+0

Isolation and Characterization of a Shigella flexneri Invasin Complex Subunit Vaccine

K. Ross Turbyfill, Antoinette B. Hartman, and Edwin V. Oaks^*

Department of Enteric Infections, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910-7500

Received 26 July 2000/Accepted 8 September 2000

The invasiveness and virulence of Shigella spp. are largely due to the expression of plasmid-encoded virulence factors, among which are the invasion plasmid antigens (Ipa proteins). After infection, the host immune response is directed primarily against lipopolysaccharide (LPS) and the virulence proteins (IpaB, IpaC, and IpaD). Recent observations have indicated that the Ipa proteins (IpaB, IpaC, and possibly IpaD) form a multiprotein complex capable of inducing the phagocytic event which internalizes the bacterium. We have isolated a complex of invasins and LPS from water-extractable antigens of virulent shigellae by ion-exchange chromatography. Western blot analysis of the complex indicates that all of the major virulence antigens of Shigella, including IpaB, IpaC, and IpaD, and LPS are components of this macromolecular complex. Mice or guinea pigs immunized intranasally with purified invasin complex (invaplex), without any additional adjuvant, mounted a significant immunoglobulin G (IgG) and IgA antibody response against the Shigella virulence antigens and LPS. The virulence-specific response was very similar to that previously noted in primates infected with shigellae. Guinea pigs (keratoconjunctivitis model) or mice (lethal lung model) immunized intranasally on days 0, 14, and 28 and challenged 3 weeks later with virulent shigellae were protected from disease (P < 0.01 for both animal models).

---

^* Corresponding author. Mailing address: Department of Enteric Infections, Walter Reed Army Institute of Research, 503 Robert Grant Ave., Silver Spring, MD 20910-7500. Phone: (301) 319-9268. Fax: (301) 319-9801. E-mail: edwin.oaks{at}na.amedd.army.mil.

---

Infection and Immunity, December 2000, p. 6624-6632, Vol. 68, No. 12
0019-9567/00/$04.00+0

This article has been cited by other articles:

• Kaminski, R. W., Turbyfill, K. R., Chao, C., Ching, W. M., Oaks, E. V. (2009). Mucosal Adjuvanticity of a Shigella Invasin Complex with DNA-Based Vaccines. CVI 16: 574-586 [Abstract] [Full Text]  
• Ivanov, M. I., Noel, B. L., Rampersaud, R., Mena, P., Benach, J. L., Bliska, J. B. (2008). Vaccination of Mice with a Yop Translocon Complex Elicits Antibodies That Are Protective against Infection with F1- Yersinia pestis. Infect. Immun. 76: 5181-5190 [Abstract] [Full Text]  
• Coburn, B., Sekirov, I., Finlay, B. B. (2007). Type III Secretion Systems and Disease. Clin. Microbiol. Rev. 20: 535-549 [Abstract] [Full Text]  
• Kaminski, R. W., Turbyfill, K. R., Oaks, E. V. (2006). Mucosal adjuvant properties of the Shigella invasin complex.. Infect. Immun. 74: 2856-2866 [Abstract] [Full Text]  
• Byrd, W., Mog, S. R., Cassels, F. J. (2003). Pathogenicity and Immune Response Measured in Mice following Intranasal Challenge with Enterotoxigenic Escherichia coli Strains H10407 and B7A. Infect. Immun. 71: 13-21 [Abstract] [Full Text]