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Infection and Immunity, December 2000, p. 6744-6749, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

Hany Sahly,1,2,* Rainer Podschun,1 Tobias A. Oelschlaeger,3 Michael Greiwe,1 Haralambos Parolis,4 David Hasty,5 Jörn Kekow,6 Uwe Ullmann,1 Itzhak Ofek,2 and Shlomo Sela2

Department of Medical Microbiology and Virology, University of Kiel, Kiel,1 Institut für Molekulare Infektionsbiologie, University of Würzburg, Würzburg,3 and Clinic of Rheumatology, University of Magdeburg, Vogelsang,6 Germany; Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel2; School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa4; and VA Medical Center and Department of Anatomy and Neurobiology, University of Tennessee, Memphis, Tennessee5

Received 2 May 2000/Returned for modification 11 July 2000/Accepted 18 September 2000

The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.

* Corresponding author. Mailing address: Department of Medical Microbiology and Virology, University of Kiel, Brunswiker Str. 4, 24105 Kiel, Germany. Phone: 494315973316. Fax: 494315973296. E-mail: sahly{at}medmicrobio.uni-kiel.de.

Infection and Immunity, December 2000, p. 6744-6749, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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