This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sahly, H.
Right arrow Articles by Sela, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sahly, H.
Right arrow Articles by Sela, S.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 6744-6749, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Capsule Impedes Adhesion to and Invasion of Epithelial Cells by Klebsiella pneumoniae

Hany Sahly,1,2,* Rainer Podschun,1 Tobias A. Oelschlaeger,3 Michael Greiwe,1 Haralambos Parolis,4 David Hasty,5 Jörn Kekow,6 Uwe Ullmann,1 Itzhak Ofek,2 and Shlomo Sela2

Department of Medical Microbiology and Virology, University of Kiel, Kiel,1 Institut für Molekulare Infektionsbiologie, University of Würzburg, Würzburg,3 and Clinic of Rheumatology, University of Magdeburg, Vogelsang,6 Germany; Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel2; School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa4; and VA Medical Center and Department of Anatomy and Neurobiology, University of Tennessee, Memphis, Tennessee5

Received 2 May 2000/Returned for modification 11 July 2000/Accepted 18 September 2000

The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.


* Corresponding author. Mailing address: Department of Medical Microbiology and Virology, University of Kiel, Brunswiker Str. 4, 24105 Kiel, Germany. Phone: 494315973316. Fax: 494315973296. E-mail: sahly{at}medmicrobio.uni-kiel.de.


Infection and Immunity, December 2000, p. 6744-6749, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Wu, M.-F., Yang, C.-Y., Lin, T.-L., Wang, J.-T., Yang, F.-L., Wu, S.-H., Hu, B.-S., Chou, T.-Y., Tsai, M.-D., Lin, C.-H., Hsieh, S.-L. (2009). Humoral Immunity against Capsule Polysaccharide Protects the Host from magA+ Klebsiella pneumoniae-Induced Lethal Disease by Evading Toll-Like Receptor 4 Signaling. Infect. Immun. 77: 615-621 [Abstract] [Full Text]  
  • Struve, C., Bojer, M., Krogfelt, K. A. (2008). Characterization of Klebsiella pneumoniae Type 1 Fimbriae by Detection of Phase Variation during Colonization and Infection and Impact on Virulence. Infect. Immun. 76: 4055-4065 [Abstract] [Full Text]  
  • Sahly, H., Navon-Venezia, S., Roesler, L., Hay, A., Carmeli, Y., Podschun, R., Hennequin, C., Forestier, C., Ofek, I. (2008). Extended-Spectrum {beta}-Lactamase Production Is Associated with an Increase in Cell Invasion and Expression of Fimbrial Adhesins in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 52: 3029-3034 [Abstract] [Full Text]  
  • Rosen, D. A., Pinkner, J. S., Jones, J. M., Walker, J. N., Clegg, S., Hultgren, S. J. (2008). Utilization of an Intracellular Bacterial Community Pathway in Klebsiella pneumoniae Urinary Tract Infection and the Effects of FimK on Type 1 Pilus Expression. Infect. Immun. 76: 3337-3345 [Abstract] [Full Text]  
  • Sahly, H., Keisari, Y., Crouch, E., Sharon, N., Ofek, I. (2008). Recognition of Bacterial Surface Polysaccharides by Lectins of the Innate Immune System and Its Contribution to Defense against Infection: the Case of Pulmonary Pathogens. Infect. Immun. 76: 1322-1332 [Full Text]  
  • Scorpio, A., Chabot, D. J., Day, W. A., O'Brien, D. K., Vietri, N. J., Itoh, Y., Mohamadzadeh, M., Friedlander, A. M. (2007). Poly-{gamma}-Glutamate Capsule-Degrading Enzyme Treatment Enhances Phagocytosis and Killing of Encapsulated Bacillus anthracis. Antimicrob. Agents Chemother. 51: 215-222 [Abstract] [Full Text]  
  • Andrian, E., Grenier, D., Rouabhia, M. (2006). Porphyromonas gingivalis-Epithelial Cell Interactions in Periodontitis. JDR 85: 392-403 [Abstract] [Full Text]  
  • Kostina, E., Ofek, I., Crouch, E., Friedman, R., Sirota, L., Klinger, G., Sahly, H., Keisari, Y. (2005). Noncapsulated Klebsiella pneumoniae Bearing Mannose-Containing O Antigens Is Rapidly Eradicated from Mouse Lung and Triggers Cytokine Production by Macrophages following Opsonization with Surfactant Protein D. Infect. Immun. 73: 8282-8290 [Abstract] [Full Text]  
  • Schembri, M. A., Blom, J., Krogfelt, K. A., Klemm, P. (2005). Capsule and Fimbria Interaction in Klebsiella pneumoniae. Infect. Immun. 73: 4626-4633 [Abstract] [Full Text]  
  • Schwan, W. R., Beck, M. T., Hultgren, S. J., Pinkner, J., Woolever, N. L., Larson, T. (2005). Down-Regulation of the kps Region 1 Capsular Assembly Operon following Attachment of Escherichia coli Type 1 Fimbriae to D-Mannose Receptors. Infect. Immun. 73: 1226-1231 [Abstract] [Full Text]  
  • Schembri, M. A., Dalsgaard, D., Klemm, P. (2004). Capsule Shields the Function of Short Bacterial Adhesins. J. Bacteriol. 186: 1249-1257 [Abstract] [Full Text]  
  • Sahly, H., Ofek, I., Podschun, R., Brade, H., He, Y., Ullmann, U., Crouch, E. (2002). Surfactant Protein D Binds Selectively to Klebsiella pneumoniae Lipopolysaccharides Containing Mannose-Rich O-Antigens. J. Immunol. 169: 3267-3274 [Abstract] [Full Text]  
  • Cortes, G., Alvarez, D., Saus, C., Alberti, S. (2002). Role of Lung Epithelial Cells in Defense against Klebsiella pneumoniae Pneumonia. Infect. Immun. 70: 1075-1080 [Abstract] [Full Text]  
  • Swiatlo, E., Champlin, F. R., Holman, S. C., Wilson, W. W., Watt, J. M. (2002). Contribution of Choline-Binding Proteins to Cell Surface Properties of Streptococcus pneumoniae. Infect. Immun. 70: 412-415 [Abstract] [Full Text]