This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morrison, S. G.
Right arrow Articles by Morrison, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morrison, S. G.
Right arrow Articles by Morrison, R. P.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 6979-6987, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunity to Murine Chlamydia trachomatis Genital Tract Reinfection Involves B Cells and CD4+ T Cells but Not CD8+ T Cells

Sandra G. Morrison,1 Hua Su,2,dagger Harlan D. Caldwell,2 and Richard P. Morrison1,*

Department of Microbiology, Montana State University, Bozeman, Montana 59717,1 and Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 598402

Received 5 June 2000/Returned for modification 24 August 2000/Accepted 25 September 2000

CD4+ T-helper type 1 (Th1) responses are essential for the resolution of a primary Chlamydia trachomatis genital tract infection; however, elements of the immune response that function in resistance to reinfection are poorly understood. Defining the mechanisms of immune resistance to reinfection is important because the elements of protective adaptive immunity are distinguished by immunological memory and high-affinity antigen recognition, both of which are crucial to the development of efficacious vaccines. Using in vivo antibody depletion of CD4+ and CD8+ T cells prior to secondary intravaginal challenge, we identified lymphocyte populations that functioned in resistance to secondary chlamydial infection of the genital tract. Depletion of either CD4+ or CD8+ T cells in immune wild-type C57BL/6 mice had a limited effect on resistance to reinfection. However, depletion of CD4+ T cells, but not CD8+ T cells, in immune B-cell-deficient mice profoundly altered the course of secondary infection. CD4-depleted B-cell-deficient mice were unable to resolve a secondary infection, shed high levels of infectious chlamydiae, and did not resolve the infection until 3 to 4 weeks following the discontinuation of anti-CD4 treatment. These findings substantiated a predominant role for CD4+ T cells in host resistance to chlamydial reinfection of the female genital tract and demonstrated that CD8+ T cells are unnecessary for adaptive immune resistance. More importantly, however, this study establishes a previously unrecognized but very significant role for B cells in resistance to chlamydial reinfection and suggests that B cells and CD4+ T cells may function synergistically in providing immunity in this model of chlamydial infection. Whether CD4+ T cells and B cells function independently or dependently is unknown, but definition of those mechanisms is fundamental to understanding optimum protective immunity and to the development of highly efficacious immunotherapies against chlamydial urogenital infections.

* Corresponding author. Mailing address: Department of Microbiology, Lewis Hall Room 109, Montana State University, Bozeman, MT 59717. Phone: (406) 994-7959. Fax: (406) 994-4926. E-mail: morrison{at}montana.edu.

dagger Present address: Laboratory of Host Defenses, Tuberculosis Research Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Md.

Infection and Immunity, December 2000, p. 6979-6987, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yu, H., Jiang, X., Shen, C., Karunakaran, K. P., Brunham, R. C. (2009). Novel Chlamydia muridarum T Cell Antigens Induce Protective Immunity against Lung and Genital Tract Infection in Murine Models. J. Immunol. 182: 1602-1608 [Abstract] [Full Text]  
  • Pal, S., Bravo, J., Peterson, E. M., de la Maza, L. M. (2008). Protection of Wild-Type and Severe Combined Immunodeficiency Mice against an Intranasal Challenge by Passive Immunization with Monoclonal Antibodies to the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein. Infect. Immun. 76: 5581-5587 [Abstract] [Full Text]  
  • Li, W., Murthy, A. K., Guentzel, M. N., Seshu, J., Forsthuber, T. G., Zhong, G., Arulanandam, B. P. (2008). Antigen-Specific CD4+ T Cells Produce Sufficient IFN-{gamma} to Mediate Robust Protective Immunity against Genital Chlamydia muridarum Infection. J. Immunol. 180: 3375-3382 [Abstract] [Full Text]  
  • Karunakaran, K. P., Rey-Ladino, J., Stoynov, N., Berg, K., Shen, C., Jiang, X., Gabel, B. R., Yu, H., Foster, L. J., Brunham, R. C. (2008). Immunoproteomic Discovery of Novel T Cell Antigens from the Obligate Intracellular Pathogen Chlamydia. J. Immunol. 180: 2459-2465 [Abstract] [Full Text]  
  • Li, W., Guentzel, M. N., Seshu, J., Zhong, G., Murthy, A. K., Arulanandam, B. P. (2007). Induction of Cross-Serovar Protection against Genital Chlamydial Infection by a Targeted Multisubunit Vaccination Approach. CVI 14: 1537-1544 [Abstract] [Full Text]  
  • Marks, E., Verolin, M., Stensson, A., Lycke, N. (2007). Differential CD28 and Inducible Costimulatory Molecule Signaling Requirements for Protective CD4+ T-Cell-Mediated Immunity against Genital Tract Chlamydia trachomatis Infection. Infect. Immun. 75: 4638-4647 [Abstract] [Full Text]  
  • Murthy, A. K., Chambers, J. P., Meier, P. A., Zhong, G., Arulanandam, B. P. (2007). Intranasal Vaccination with a Secreted Chlamydial Protein Enhances Resolution of Genital Chlamydia muridarum Infection, Protects against Oviduct Pathology, and Is Highly Dependent upon Endogenous Gamma Interferon Production. Infect. Immun. 75: 666-676 [Abstract] [Full Text]  
  • Murthy, A. K., Cong, Y., Murphey, C., Guentzel, M. N., Forsthuber, T. G., Zhong, G., Arulanandam, B. P. (2006). Chlamydial Protease-Like Activity Factor Induces Protective Immunity against Genital Chlamydial Infection in Transgenic Mice That Express the Human HLA-DR4 Allele. Infect. Immun. 74: 6722-6729 [Abstract] [Full Text]  
  • Roan, N. R., Gierahn, T. M., Higgins, D. E., Starnbach, M. N. (2006). Monitoring the T cell response to genital tract infection. Proc. Natl. Acad. Sci. USA 103: 12069-12074 [Abstract] [Full Text]  
  • Pal, S., Peterson, E. M., de la Maza, L. M. (2005). Vaccination with the Chlamydia trachomatis Major Outer Membrane Protein Can Elicit an Immune Response as Protective as That Resulting from Inoculation with Live Bacteria. Infect. Immun. 73: 8153-8160 [Abstract] [Full Text]  
  • Morrison, S. G., Morrison, R. P. (2005). A Predominant Role for Antibody in Acquired Immunity to Chlamydial Genital Tract Reinfection. J. Immunol. 175: 7536-7542 [Abstract] [Full Text]  
  • Morrison, S. G., Morrison, R. P. (2005). The Protective Effect of Antibody in Immunity to Murine Chlamydial Genital Tract Reinfection Is Independent of Immunoglobulin A. Infect. Immun. 73: 6183-6186 [Abstract] [Full Text]  
  • Gupta, S., Janani, R., Bin, Q., Luciw, P., Greer, C., Perri, S., Legg, H., Donnelly, J., Barnett, S., O'Hagan, D., Polo, J. M., Vajdy, M. (2005). Characterization of Human Immunodeficiency Virus Gag-Specific Gamma Interferon-Expressing Cells following Protective Mucosal Immunization with Alphavirus Replicon Particles. J. Virol. 79: 7135-7145 [Abstract] [Full Text]  
  • Rey-Ladino, J., Koochesfahani, K. M., Zaharik, M. L., Shen, C., Brunham, R. C. (2005). A Live and Inactivated Chlamydia trachomatis Mouse Pneumonitis Strain Induces the Maturation of Dendritic Cells That Are Phenotypically and Immunologically Distinct. Infect. Immun. 73: 1568-1577 [Abstract] [Full Text]  
  • Burton, M. J., Bailey, R. L., Jeffries, D., Mabey, D. C. W., Holland, M. J. (2004). Cytokine and Fibrogenic Gene Expression in the Conjunctivas of Subjects from a Gambian Community Where Trachoma Is Endemic. Infect. Immun. 72: 7352-7356 [Abstract] [Full Text]  
  • Steele, L. N., Balsara, Z. R., Starnbach, M. N. (2004). Hematopoietic Cells Are Required to Initiate a Chlamydia trachomatis-Specific CD8+ T Cell Response. J. Immunol. 173: 6327-6337 [Abstract] [Full Text]  
  • Maxion, H. K., Liu, W., Chang, M.-H., Kelly, K. A. (2004). The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection. Infect. Immun. 72: 6330-6340 [Abstract] [Full Text]  
  • Berry, L. J., Hickey, D. K., Skelding, K. A., Bao, S., Rendina, A. M., Hansbro, P. M., Gockel, C. M., Beagley, K. W. (2004). Transcutaneous Immunization with Combined Cholera Toxin and CpG Adjuvant Protects against Chlamydia muridarum Genital Tract Infection. Infect. Immun. 72: 1019-1028 [Abstract] [Full Text]  
  • Buendia, A. J., Del Rio, L., Ortega, N., Sanchez, J., Gallego, M. C., Caro, M. R., Navarro, J. A., Cuello, F., Salinas, J. (2002). B-Cell-Deficient Mice Show an Exacerbated Inflammatory Response in a Model of Chlamydophila abortus Infection. Infect. Immun. 70: 6911-6918 [Abstract] [Full Text]  
  • Pal, S., Davis, H. L., Peterson, E. M., de la Maza, L. M. (2002). Immunization with the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein by Use of CpG Oligodeoxynucleotides as an Adjuvant Induces a Protective Immune Response against an Intranasal Chlamydial Challenge. Infect. Immun. 70: 4812-4817 [Abstract] [Full Text]  
  • Hawkins, R. A., Rank, R. G., Kelly, K. A. (2002). A Chlamydia trachomatis-Specific Th2 Clone Does Not Provide Protection against a Genital Infection and Displays Reduced Trafficking to the Infected Genital Mucosa. Infect. Immun. 70: 5132-5139 [Abstract] [Full Text]  
  • Morrison, R. P., Caldwell, H. D. (2002). Immunity to Murine Chlamydial Genital Infection. Infect. Immun. 70: 2741-2751 [Full Text]  
  • Shaw, J., Grund, V., Durling, L., Crane, D., Caldwell, H. D. (2002). Dendritic Cells Pulsed with a Recombinant Chlamydial Major Outer Membrane Protein Antigen Elicit a CD4+ Type 2 Rather than Type 1 Immune Response That Is Not Protective. Infect. Immun. 70: 1097-1105 [Abstract] [Full Text]  
  • Pal, S., Theodor, I., Peterson, E. M., de la Maza, L. M. (2001). Immunization with the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein Can Elicit a Protective Immune Response against a Genital Challenge. Infect. Immun. 69: 6240-6247 [Abstract] [Full Text]  
  • Shaw, J. H., Grund, V. R., Durling, L., Caldwell, H. D. (2001). Expression of Genes Encoding Th1 Cell-Activating Cytokines and Lymphoid Homing Chemokines by Chlamydia-Pulsed Dendritic Cells Correlates with Protective Immunizing Efficacy. Infect. Immun. 69: 4667-4672 [Abstract] [Full Text]  
  • Morrison, S. G., Morrison, R. P. (2001). Resolution of Secondary Chlamydia trachomatis Genital Tract Infection in Immune Mice with Depletion of Both CD4+ and CD8+ T cells. Infect. Immun. 69: 2643-2649 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»