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Infection and Immunity, December 2000, p. 7018-7027, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pyelonephritogenic Diffusely Adhering Escherichia coli EC7372 Harboring Dr-II Adhesin Carries Classical Uropathogenic Virulence Genes and Promotes Cell Lysis and Apoptosis in Polarized Epithelial Caco-2/TC7 Cells

Julie Guignot,1,2 Jacqueline Breard,2,3 Marie-Françoise Bernet-Camard,1,2 Isabelle Peiffer,1,2 Bogdan J. Nowicki,4 Alain L. Servin,1,2 and Anne-Beatrice Blanc-Potard1,2,*

Unité 5101 and Unité 461,3 Faculté de Pharmacie Paris XI, Institut National de la Santé et de la Recherche Médicale, and Faculté de Pharmacie Paris XI, Institut Fédératif de Recherche IFR75,2 F-92296 Châtenay-Malabry, France, and Department of Obstetrics & Gynecology and Department of Microbiology & Immunology, The University of Texas Medical Branch, Galveston, Texas 775504

Received 28 June 2000/Returned for modification 5 September 2000/Accepted 19 September 2000

Diffusely adhering Escherichia coli (DAEC) strains expressing adhesins of the Afa/Dr family bind to epithelial cells in a diffuse adherence pattern by recognizing a common receptor, the decay-accelerating factor (CD55). Recently, a novel CD55-binding adhesin, named Dr-II, was identified from the pyelonephritogenic strain EC7372. In this report, we show that despite the low level of sequence identity between Dr-II and other members of the Afa/Dr family, EC7372 induces pathophysiological effects similar to those induced by other Afa/Dr DAEC strains on the polarized epithelial cell line Caco-2/TC7. Specifically, the Dr-II adhesin was sufficient to promote CD55 and CD66e clustering around adhering bacteria and apical cytoskeleton rearrangements. Unlike other Afa/Dr DAEC strains, EC7372 expresses a functional hemolysin that promotes a rapid cellular lysis. In addition, cell death by apoptosis or necrosis was observed in EC7372-infected Caco-2/TC7 cells, depending on infection time. Our results indicate that EC7372 harbors a pathogenicity island (PAI) similar to the one described for the pyelonephritogenic strain CFT073, which carries both hly and pap operons. Cumulatively, our findings indicate that strain EC7372 can be considered a prototype of a subclass of Afa/Dr DAEC isolates that have acquired a PAI harboring several classical uropathogenic virulence genes.

* Corresponding author. Mailing address: INSERM Unité 510, UFR de Pharmacie Paris XI, F-92296 Châtenay-Malabry, France. Phone: 33.1.46.83.58.43. Fax: 33.1.46.83.58.44. E-mail: anne.blanc{at}cep.u-psud.fr.

Infection and Immunity, December 2000, p. 7018-7027, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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