This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, B.
Right arrow Articles by Haas, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, B.
Right arrow Articles by Haas, A.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 7039-7048, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phagosome Acidification Has Opposite Effects on Intracellular Survival of Bordetella pertussis and B. bronchiseptica

Boris Schneider, Roy Gross,* and Albert Haas

Lehrstuhl für Mikrobiologie, Biozentrum der Universität Würzburg, D-97074 Würzburg, Germany

Received 18 July 2000/Returned for modification 28 August 2000/Accepted 26 September 2000

Bordetella pertussis is readily killed after uptake by professional phagocytes, whereas its close relative Bordetella bronchiseptica is not and can persist intracellularly for days. Phagocytosis of members of either species by a mouse macrophage cell line results in transport of the bacteria to a phagosomal compartment positive for the lysosome-associated membrane protein 1, the protease cathepsin D, and the late endosomal vacuolar proton-pumping ATPase but negative for the early endosome antigen 1 and the early endosomal transferrin receptor. In addition, we demonstrate that Bordetella-containing phagosomes rapidly acidify to pH 4.5 to 5.0. Taken together, these data demonstrate that Bordetella-containing phagosomes rapidly mature to an acidic late endosomal/lysosomal compartment. Following up on this observation, we determined that B. pertussis does not survive in bacterial growth media adjusted to a pH of 4.5, whereas this pH has only minor effects on the growth of B. bronchiseptica. Raising the intracellular pH in infected macrophages by the addition of bafilomycin A1, ammonium chloride, or monensin increases the survival of acid-sensitive B. pertussis but, surprisingly, decreases that of acid-tolerant B. bronchiseptica. In summary, we hypothesize that the differential survival of B. pertussis and B. bronchiseptica in macrophages is, at least in part, due to the differences in their acid tolerance.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Biozentrum, Am Hubland, D-97074 Würzburg, Germany. Phone: (931) 888 4403. Fax: (931) 888 4402. E-mail: roy{at}biozentrum.uni-wuerzburg.de.

Infection and Immunity, December 2000, p. 7039-7048, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kamanova, J., Kofronova, O., Masin, J., Genth, H., Vojtova, J., Linhartova, I., Benada, O., Just, I., Sebo, P. (2008). Adenylate Cyclase Toxin Subverts Phagocyte Function by RhoA Inhibition and Unproductive Ruffling. J. Immunol. 181: 5587-5597 [Abstract] [Full Text]  
  • Schwartz, J. T., Allen, L.-A. H. (2006). Role of urease in megasome formation and Helicobacter pylori survival in macrophages. J. Leukoc. Biol. 79: 1214-1225 [Abstract] [Full Text]  
  • Newman, S. L., Gootee, L., Hilty, J., Morris, R. E. (2006). Human Macrophages Do Not Require Phagosome Acidification to Mediate Fungistatic/Fungicidal Activity against Histoplasma capsulatum. J. Immunol. 176: 1806-1813 [Abstract] [Full Text]  
  • Stubs, D., Fuchs, T. M., Schneider, B., Bosserhoff, A., Gross, R. (2005). Identification and regulation of cold-inducible factors of Bordetella bronchiseptica. Microbiology 151: 1895-1909 [Abstract] [Full Text]  
  • Fedele, G., Stefanelli, P., Spensieri, F., Fazio, C., Mastrantonio, P., Ausiello, C. M. (2005). Bordetella pertussis-Infected Human Monocyte-Derived Dendritic Cells Undergo Maturation and Induce Th1 Polarization and Interleukin-23 Expression. Infect. Immun. 73: 1590-1597 [Abstract] [Full Text]  
  • Rodriguez, M. E., Hellwig, S. M. M., Hozbor, D. F., Leusen, J., van der Pol, W. -L., van de Winkel, J. G. J. (2001). Fc Receptor-Mediated Immunity Against Bordetella pertussis. J. Immunol. 167: 6545-6551 [Abstract] [Full Text]  
  • Luhrmann, A., Streker, K., Schuttfort, A., Daniels, J. J. D., Haas, A. (2001). Afipia felis induces uptake by macrophages directly into a nonendocytic compartment. Proc. Natl. Acad. Sci. USA 10.1073/pnas.121190398v1 [Abstract] [Full Text]  
  • Luhrmann, A., Streker, K., Schuttfort, A., Daniels, J. J. D., Haas, A. (2001). Afipia felis induces uptake by macrophages directly into a nonendocytic compartment. Proc. Natl. Acad. Sci. USA 98: 7271-7276 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»