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Infection and Immunity, December 2000, p. 7069-7077, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

OppA of Listeria monocytogenes, an Oligopeptide-Binding Protein Required for Bacterial Growth at Low Temperature and Involved in Intracellular Survival

Elise Borezee, Elisabeth Pellegrini, and Patrick Berche*

Inserm U411, Faculté de Médecine Necker, 75730 Paris Cedex 15, France

Received 19 July 2000/Returned for modification 17 August 2000/Accepted 6 September 2000

We identified a new oligopeptide permease operon in the pathogen Listeria monocytogenes. This opp operon consists of five genes (oppA, oppB, oppC, oppD, and oppF) and displays the same genetic organization as those of several bacterial species. The first gene of this operon, oppA, encodes a 62-kDa protein sharing 33% identity with OppA of Bacillus subtilis and is expressed predominantly during exponential growth. The function of oppA was studied by constructing an oppA deletion mutant. The phenotype analysis of this mutant revealed that OppA mediates the transport of oligopeptides and is required for bacterial growth at low temperature. The wild-type phenotype was restored by complementing the mutant with oppA. We also found that OppA is involved in intracellular survival in macrophages and in bacterial growth in organs of mice infected with L. monocytogenes, although the level of virulence was not altered in the mutant. These results show the major role of OppA in the uptake of oligopeptides and the pleiotropic effects of this oligopeptide-binding protein on the behavior of this pathogen in the environment and in its host.

* Corresponding author. Mailing address: INSERM U411, Faculté de Médecine Necker, 156, rue de Vaugirard, 75730 Paris Cedex 15, France. Phone: [33] 1 40 61 53 71. Fax: [33] 1 40 61 55 92. E-mail: berche{at}necker.fr.

Infection and Immunity, December 2000, p. 7069-7077, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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