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Infection and Immunity, December 2000, p. 7137-7140, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

DNA Rearrangements in the Flagellin Locus of an flaA Mutant of Campylobacter jejuni during Colonization of Chicken Ceca

Piet J. M. Nuijten,1,* Anton J. G. van den Berg,1 Ivan Formentini,1 Bernard A. M. van der Zeijst,2,dagger and Anton A. C. Jacobs1

Intervet International b.v., 5830 AA Boxmeer,1 and Department of Bacteriology, Faculty of Veterinary Medicine, Universiteit Utrecht, Utrecht,2 The Netherlands

Received 9 March 2000/Returned for modification 8 May 2000/Accepted 28 August 2000

Campylobacter jejuni is an enteropathogen for humans but commensal for chickens. In both hosts, the flagella and motility are important colonization factors. The flagellin gene is duplicated in Campylobacter, but only one flagellin gene, flaA, is sufficient for motility. We found that, during colonization of the chicken intestine, a nonmotile flaA mutant of C. jejuni underwent rearrangements within its flagellin locus, thereby regaining its motility and colonization capacity. In contrast, in vitro motile revertants isolated from liquid culture showed different flagellin DNA rearrangements than after reversion in the chicken.

* Corresponding author. Mailing address: Bacteriological R&D, Intervet International BV, P.O. Box 31, 5830 AA Boxmeer, The Netherlands. Phone: 31(0)485-587326. Fax: 31(0)485-587490. E-mail: Piet.nuijten{at}intervet.com.

dagger Present address: National Institute of Public Health, 3720 BA Bilthoven, The Netherlands.

Infection and Immunity, December 2000, p. 7137-7140, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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