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Infection and Immunity, December 2000, p. 7141-7143, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antigenicity, Expression, and Molecular Characterization of Surface-Located Pullulanase of Streptococcus pneumoniae

Roger J. M. Bongaerts,dagger Hans-Peter Heinz, Ulrich Hadding, and Gregor Zysk*

Department of Medical Microbiology and Virology, University of Duesseldorf, Duesseldorf, Germany

Received 24 April 2000/Returned for modification 5 June 2000/Accepted 20 September 2000

A putative pullulanase-encoding gene from Streptococcus pneumoniae was identified by screening a genomic expression library with human convalescent-phase serum. The 3,864-bp gene encoded a 143-kDa protein. Surface location and pullulanase activity of the protein, designated SpuA, was demonstrated. SpuA was present in all investigated pneumococcal isolates of different serotypes. The spuA 5' end was highly conserved among clinical isolates except for a 75-bp region. The properties of SpuA reported here indicate that this novel immunogenic surface protein might have potential as a vaccine target.

* Corresponding author. Mailing address: Department of Medical Microbiology and Virology, University of Duesseldorf, Geb. 22.21/U1, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany. Phone and fax: 49-211-811 24 96. E-mail: zysk{at}uni-duesseldorf.de.

dagger Present address: Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.

Infection and Immunity, December 2000, p. 7141-7143, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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