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Infection and Immunity, December 2000, p. 7144-7148, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Human CD8+ T Cells Specific for Mycobacterium tuberculosis Secreted Antigens in Tuberculosis Patients and Healthy BCG-Vaccinated Controls in The Gambia

Steven M. Smith,1,* Michèl R. Klein,2 Adam S. Malin,1 Jackson Sillah,2 Kris Huygen,3 Peter Andersen,4 Keith P. W. J. McAdam,2 and Hazel M. Dockrell1

Immunology Unit, London School of Hygiene & Tropical Medicine, London, United Kingdom1; Tuberculosis Research Programme, MRC Laboratories, Fajara, The Gambia2; Mycobacterial Immunology, Pasteur Institute of Brussels, Brussels, Belgium3; and Department of Tuberculosis Immunology, Statens Serum Institut, Copenhagen, Denmark4

Received 19 May 2000/Returned for modification 6 July 2000/Accepted 28 August 2000

Intracellular flow cytometry analysis of perforin production by CD8+ T cells showed levels were greatly reduced in tuberculosis (TB) patients compared to healthy controls. Reduced cytotoxic-T-lymphocyte activity was also obtained with CD8+ T cells from TB patients compared to healthy controls in The Gambia. A change in antigen recognition was noted between the two groups of donors: in addition to recognition of Ag85A and Ag85B, as seen in healthy donors, a prominent ESAT-6 response was found in TB patients.

* Corresponding author. Mailing address: Immunology Unit, London School of Hygiene & Tropical Medicine, Keppel Street, London, WC1E 7HT, United Kingdom. Phone: 44 (0) 20-7927-2832. Fax: 44 (0) 20-7637-4314. E-mail: steven.smith{at}lshtm.ac.uk.

Infection and Immunity, December 2000, p. 7144-7148, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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