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Infection and Immunity, December 2000, p. 7166-7171, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

fbpABC Gene Cluster in Neisseria meningitidis Is Transcribed as an Operon

Heng H. Khun,1 Vinay Deved,1 Howard Wong,2 and B. Craig Lee1,*

Department of Microbiology and Infectious Disease, University of Calgary, Calgary, Alberta, Canada T2N 4N1,1 and Department of Pathology and Laboratory Medicine, Calgary Laboratory Services, Foothills Medical Centre, Calgary, Alberta, Canada T2N 2T92

Received 30 June 2000/Returned for modification 11 August 2000/Accepted 15 September 2000

The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with an fbpC-sequence-specific oligonucleotide indicates that fbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.

* Corresponding author. Present address: Division of Infectious Diseases, Department of Medicine, University of Ottawa, 501 Smyth Rd., Ottawa, Ontario, Canada K1H 8L6. Phone: (613) 737-8880. Fax: (613) 737-8925. E-mail: clee{at}uottawa.ca.

Infection and Immunity, December 2000, p. 7166-7171, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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