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Infection and Immunity, December 2000, p. 7172-7174, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interleukin 12 as an Adjuvant to WI-1 Adhesin Immunization Augments Delayed-Type Hypersensitivity, Shifts the Subclass Distribution of Immunoglobulin G Antibodies, and Enhances Protective Immunity to Blastomyces dermatitidis Infection

Marcel Wüthrich,1 Beatriz E. Finkel-Jiminez,1 and Bruce S. Klein1,2,3,4,*

Departments of Pediatrics,1 Internal Medicine,2 and Medical Microbiology and Immunology3 and the Comprehensive Cancer Center,4 University of Wisconsin Medical School, University of Wisconsin Hospital and Clinics, Madison, Wisconsin 53792

Received 28 June 2000/Returned for modification 7 August 2000/Accepted 30 August 2000

Cell-mediated immunity is pivotal in host resistance to Blastomyces dermatitidis infection. Immunization of mice with the WI-1 adhesin enhances resistance against experimental pulmonary infection but elicits features of a mixed T-helper-cell immune response. Immune mice acquire delayed-type hypersensitivity (DTH) but also high titers of WI-1-specific immunoglobulin G1 (IgG1) and IgG2b, a result indicative of T-helper-2 cellular immunity. We report that interleukin-12, used as an adjuvant for WI-1 immunization, augments DTH, shifts the balance of the T-helper phenotype toward Th1, and enhances resistance to B. dermatitidis infection.

* Corresponding author. Mailing address: University of Wisconsin Hospitals and Clinics, 600 Highland Ave., K4/434, Madison, WI 53792. Phone: (608) 263-9217. Fax: (608) 263-0440. E-mail: bsklein{at}facstaff.wisc.edu.

Infection and Immunity, December 2000, p. 7172-7174, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Su, Z., Stevenson, M. M. (2002). IL-12 Is Required for Antibody-Mediated Protective Immunity Against Blood-Stage Plasmodiumchabaudi AS Malaria Infection in Mice. J. Immunol. 168: 1348-1355 [Abstract] [Full Text]  


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