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Infection and Immunity, December 2000, p. 7180-7185, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phylogeny of Vibrio cholerae Based on recA Sequence

O. Colin Stine,1,2 Shanmuga Sozhamannan,1,3 Qing Gou,2 Siqen Zheng,2 J. Glenn Morris Jr.,1,3,* and Judith A. Johnson1,3,4

Departments of Epidemiology and Preventive Medicine1 and Pathology4 and Department of Pediatrics and Center for the Study of Asthma and Other Complex Diseases,2 University of Maryland School of Medicine, and Veterans Affairs Maryland Health Care System,3 Baltimore, Maryland 21201

Received 10 July 2000/Returned for modification 17 July 2000/Accepted 7 September 2000

We sequenced a 705-bp fragment of the recA gene from 113 Vibrio cholerae strains and closely related species. One hundred eighty-seven nucleotides were phylogenetically informative, 55 were phylogenetically uninformative, and 463 were invariant. Not unexpectedly, Vibrio parahaemolyticus and Vibrio vulnificus strains formed out-groups; we also identified isolates which resembled V. cholerae biochemically but which did not cluster with V. cholerae. In many instances, V. cholerae serogroup designations did not correlate with phylogeny, as reflected by recA sequence divergence. This observation is consistent with the idea that there is horizontal transfer of O-antigen biosynthesis genes among V. cholerae strains.

* Corresponding author. Mailing address: Rm. 934, Medical School Teaching Facility, University of Maryland School of Medicine, 10 S. Pine St., Baltimore, MD 21201. Phone: (410) 706-4580. Fax: (410) 706-4581. E-mail: jmorris{at}medicine.umaryland.edu.

Infection and Immunity, December 2000, p. 7180-7185, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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