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Infection and Immunity, December 2000, p. 7202-7208, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Localization of Dysfunctional Tight Junctions in Salmonella enterica Serovar Typhimurium-Infected Epithelial Layers

Mark A. Jepson,1,* Hélène B. Schlecht,1,dagger and Carla B. Collares-Buzato2

Cell Imaging Facility and Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom,1 and Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil2

Received 3 August 2000/Accepted 15 September 2000

Infection of polarized MDCK epithelial layers by Salmonella enterica serovar Typhimurium is accompanied by increased tight junction permeability and by contraction of perijunctional actinomyosin. We localized dysfunctional tight junctions in serovar Typhimurium-infected MDCK layers by imaging apical-basolateral intramembrane diffusion of fluorescent lipid and found that loss of the apical-basolateral diffusion barrier (tight junction fence function) was most marked in areas of prominent perijunctional contraction. The protein kinase inhibitor staurosporine prevented perijunctional contraction but did not reverse the effects of serovar Typhimurium on tight junction barrier function. Hence, perijunctional contraction is not required for Salmonella-induced tight junction dysfunction and this epithelial response to infection may be multifactorial.

* Corresponding author. Mailing address: Cell Imaging Facility and Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, United Kingdom. Phone: 44 117 928 7410. Fax: 44 117 928 8274. E-mail: m.a.jepson{at}bristol.ac.uk.

dagger Present address: Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom.

Infection and Immunity, December 2000, p. 7202-7208, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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