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Infection and Immunity, February 2000, p. 518-525, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Defective Hyphal Development and Avirulence Caused by a Deletion of the SSK1 Response Regulator Gene in Candida albicans

José Antonio Calera,* Xiao-Jiong Zhao, and Richard Calderone

Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007-2197

Received 27 July 1999/Returned for modification 1 September 1999/Accepted 26 October 1999

In a previous study, we reported the isolation and characterization of the two-component response regulator SSK1 gene of Candida albicans. This gene is a structural but not a functional homolog of the SSK1 and mcs4+ genes of Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. In the present study, we have constructed and phenotypically characterized Delta ssk1 mutants of C. albicans. The results confirmed our previous observation that CaSSK1, unlike SSK1 or mcs4+, does not regulate cellular responses to either osmotic or oxidative stress. Instead, Delta ssk1 null strains showed severely reduced hyphal formation on serum agar and were totally defective in hyphal development on other solid media, such as medium 199 (pH 7.5) and Spider medium. In contrast, under conditions of low nitrogen availability on solid media, Delta ssk1 null strains dramatically hyperinvaded the agar. However, while forming germ tubes and hyphae in liquid media similar to those of the wild type, Delta ssk1 null strains flocculated in a manner similar to that of Delta chk1 two-component histidine kinase mutants, which we have previously described. Finally, virulence studies indicated that SSK1 is essential for the pathogenesis of C. albicans, suggesting that the Ssk1p response regulator could be a good target for antifungal therapy.

* Corresponding author. Mailing address: Department of Microbiology, Med-Dent Building, Lab SE303, Georgetown University Medical Center, 3900 Reservoir Rd., N.W., Washington, DC 20007-2197. Phone: (202) 687-1796. Fax: (202) 687-1800. E-mail: abadj{at}gusun.georgetown.edu.

Infection and Immunity, February 2000, p. 518-525, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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