This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cho, N.-H.
Right arrow Articles by Kim, I.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, N.-H.
Right arrow Articles by Kim, I.-S.

 Previous Article  |  Next Article 

Infection and Immunity, February 2000, p. 594-602, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of Chemokine Genes in Murine Macrophages Infected with Orientia tsutsugamushi

Nam-Hyuk Cho,1 Seung-Yong Seong,1,* Myung-Suk Huh,1 Tae-Hee Han,2 Young-Sang Koh,3 Myung-Sik Choi,1 and Ik-Sang Kim1

Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799,1 Department of Microbiology and Immunology, Sungkyunkwan University School of Medicine, Suwon 440-746,2 and Department of Microbiology, Cheju National University College of Medicine, Cheju 690-756,3 Republic of Korea

Received 9 August 1999/Returned for modification 10 September 1999/Accepted 4 November 1999

Scrub typhus, caused by Orientia tsutsugamushi infection, is characterized by local as well as systemic inflammatory manifestations. Inflammation is initiated by O. tsutsugamushi-infected macrophages and endothelial cells in the dermis. We investigated the regulation of chemokine induction in macrophage cell line J774A.1 in response to O. tsutsugamushi infection. The mRNAs for macrophage inflammatory proteins 1alpha /beta (MIP-1alpha /beta ), MIP-2, and macrophage chemoattractant protein 1 were induced within 30 min, and their levels showed a transitory peak for 3 to 12 h. However, the lymphotactin, eotaxin, gamma interferon-inducible protein 10, and T-cell activation gene 3 mRNAs were not detected by RNase protection assays. Heat-killed O. tsutsugamushi induced a similar extent of chemokine responses. Induction of the chemokine genes was not blocked by the eukaryotic protein synthesis inhibitor cycloheximide, suggesting that de novo synthesis of host cell protein is not required for these transcriptional responses. The induction of chemokine mRNAs by O. tsutsugamushi was blocked by the inhibitors of NF-kappa B activation. Furthermore, O. tsutsugamushi induced the nuclear translocation and activation of NF-kappa B. These results demonstrate that heat-stable molecules of O. tsutsugamushi induce a subset of chemokine genes and that induction involves activation of the transcription factor NF-kappa B.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea. Phone: 82-2-740-8301. Fax: 82-2-743-0881. E-mail: seongsy{at}plaza.snu.ac.kr.

Infection and Immunity, February 2000, p. 594-602, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Drevets, D. A., Leenen, P. J. M., Greenfield, R. A. (2004). Invasion of the Central Nervous System by Intracellular Bacteria. Clin. Microbiol. Rev. 17: 323-347 [Abstract] [Full Text]  
  • MORIUCHI, M., TAMURA, A., MORIUCHI, H. (2003). IN VITRO REACTIVATION OF HUMAN IMMUNODEFICIENCY VIRUS-1 UPON STIMULATION WITH SCRUB TYPHUS RICKETTSIAL INFECTION. Am J Trop Med Hyg 68: 557-561 [Abstract] [Full Text]  
  • Jaramillo, M., Olivier, M. (2002). Hydrogen Peroxide Induces Murine Macrophage Chemokine Gene Transcription Via Extracellular Signal-Regulated Kinase- and Cyclic Adenosine 5'-Monophosphate (cAMP)-Dependent Pathways: Involvement of NF-{kappa}B, Activator Protein 1, and cAMP Response Element Binding Protein. J. Immunol. 169: 7026-7038 [Abstract] [Full Text]  
  • Gao, J. J., Diesl, V., Wittmann, T., Morrison, D. C., Ryan, J. L., Vogel, S. N., Follettie, M. T. (2002). Regulation of gene expression in mouse macrophages stimulated with bacterial CpG-DNA and lipopolysaccharide. J. Leukoc. Biol. 72: 1234-1245 [Abstract] [Full Text]  
  • Cho, N.-H., Seong, S.-Y., Huh, M.-S., Kim, N.-H., Choi, M.-s., Kim, I.-s. (2002). Induction of the Gene Encoding Macrophage Chemoattractant Protein 1 by Orientia tsutsugamushi in Human Endothelial Cells Involves Activation of Transcription Factor Activator Protein 1. Infect. Immun. 70: 4841-4850 [Abstract] [Full Text]  
  • Kim, M.-K., Seong, S.-Y., Seoh, J.-Y., Han, T.-H., Song, H.-J., Lee, J.-E., Shin, J.-H., Lim, B.-U., Kang, J.-S. (2002). Orientia tsutsugamushi Inhibits Apoptosis of Macrophages by Retarding Intracellular Calcium Release. Infect. Immun. 70: 4692-4696 [Abstract] [Full Text]  
  • Caamano, J., Hunter, C. A. (2002). NF-{kappa}B Family of Transcription Factors: Central Regulators of Innate and Adaptive Immune Functions. Clin. Microbiol. Rev. 15: 414-429 [Abstract] [Full Text]  
  • Foxman, E. F., Zhang, M., Hurst, S. D., Muchamuel, T., Shen, D., Wawrousek, E. F., Chan, C.-C., Gery, I. (2002). Inflammatory Mediators in Uveitis: Differential Induction of Cytokines and Chemokines in Th1- Versus Th2-Mediated Ocular Inflammation. J. Immunol. 168: 2483-2492 [Abstract] [Full Text]  
  • Morimoto, K., Amano, H., Sonoda, F., Baba, M., Senba, M., Yoshimine, H., Yamamoto, H., Ii, T., Oishi, K., Nagatake, T. (2001). Alveolar Macrophages that Phagocytose Apoptotic Neutrophils Produce Hepatocyte Growth Factor during Bacterial Pneumonia in Mice. Am. J. Respir. Cell Mol. Bio. 24: 608-615 [Abstract] [Full Text]  
  • Cho, N.-H., Seong, S.-Y., Choi, M.-S., Kim, I.-S. (2001). Expression of Chemokine Genes in Human Dermal Microvascular Endothelial Cell Lines Infected with Orientia tsutsugamushi. Infect. Immun. 69: 1265-1272 [Abstract] [Full Text]  
  • Kim, S.-W., Ihn, K.-S., Han, S.-H., Seong, S.-Y., Kim, I.-S., Choi, M.-S. (2001). Microtubule- and Dynein-Mediated Movement of Orientia tsutsugamushi to the Microtubule Organizing Center. Infect. Immun. 69: 494-500 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»