This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neth, O.
Right arrow Articles by Turner, M. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neth, O.
Right arrow Articles by Turner, M. W.

 Previous Article  |  Next Article 

Infection and Immunity, February 2000, p. 688-693, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mannose-Binding Lectin Binds to a Range of Clinically Relevant Microorganisms and Promotes Complement Deposition

Olaf Neth,1 Dominic L. Jack,1 Alister W. Dodds,2 Helen Holzel,3 Nigel J. Klein,1 and Malcolm W. Turner1,*

Immunobiology Unit, Institute of Child Health, University College London,1 and Department of Microbiology, Great Ormond Street Hospital for Children,3 NHS Trust, London, and Immunochemistry Unit, Medical Research Council, Oxford,2 United Kingdom

Received 6 August 1999/Returned for modification 7 October 1999/Accepted 7 November 1999

Mannose-binding lectin (MBL) is a collagenous serum lectin believed to be of importance in innate immunity. Genetically determined low levels of the protein are known to predispose to infections. In this study the binding of purified MBL to pathogens isolated from immunocompromised children was investigated by flow cytometry. Diverse Candida species, Aspergillus fumigatus, Staphylococcus aureus, and beta-hemolytic group A streptococci exhibited strong binding of MBL, whereas Escherichia coli, Klebsiella species, and Haemophilus influenzae type b were characterized by heterogeneous binding patterns. In contrast, beta-hemolytic group B streptococci, Streptococcus pneumoniae, and Staphylococcus epidermidis showed low levels of binding. Bound MBL was able to promote C4 deposition in a concentration-dependent manner. We conclude that MBL may be of importance in first-line immune defense against several important pathogens.

* Corresponding author. Mailing address: Immunobiology Unit, Institute of Child Health, 30 Guilford St., London, United Kingdom WC1N 1EH. Phone: 0044-171-2079-052215. Fax: 0044-2078-8138494. E-mail: mturner{at}ich.ucl.ac.uk.

Infection and Immunity, February 2000, p. 688-693, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ma, Y. J., Doni, A., Hummelshoj, T., Honore, C., Bastone, A., Mantovani, A., Thielens, N. M., Garred, P. (2009). Synergy between Ficolin-2 and Pentraxin 3 Boosts Innate Immune Recognition and Complement Deposition. J. Biol. Chem. 284: 28263-28275 [Abstract] [Full Text]  
  • Park, S. J., Mehrad, B. (2009). Innate Immunity to Aspergillus Species. Clin. Microbiol. Rev. 22: 535-551 [Abstract] [Full Text]  
  • Zuo, D.-M., Zhang, L.-Y., Lu, X., Liu, Y., Chen, Z.-L. (2009). Protective role of mouse MBL-C on intestinal mucosa during Shigella flexneri invasion. Int Immunol 21: 1125-1134 [Abstract] [Full Text]  
  • Dagenais, T. R. T., Keller, N. P. (2009). Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis. Clin. Microbiol. Rev. 22: 447-465 [Abstract] [Full Text]  
  • Oliva, A.-M., Pavan, P. R., Margo, C. E., Pautler, S. E. (2009). Recalcitrant Candida Endophthalmitis Associated With Mannose-Binding Lectin Deficiency. Arch Ophthalmol 127: 822-823 [Full Text]  
  • Norup, L. R., Dalgaard, T. S., Friggens, N. C., Sorensen, P., Juul-Madsen, H. R. (2009). Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli. Poult. Sci. 88: 543-553 [Abstract] [Full Text]  
  • Fidler, K J, Hilliard, T N, Bush, A, Johnson, M, Geddes, D M, Turner, M W, Alton, E W F W, Klein, N J, Davies, J C (2009). Mannose-binding lectin is present in the infected airway: a possible pulmonary defence mechanism. Thorax 64: 150-155 [Abstract] [Full Text]  
  • Endeman, H., Herpers, B. L., de Jong, B. A. W., Voorn, G. P., Grutters, J. C., van Velzen-Blad, H., Biesma, D. H. (2008). Mannose-Binding Lectin Genotypes in Susceptibility to Community-Acquired Pneumonia. Chest 134: 1135-1140 [Abstract] [Full Text]  
  • Dumestre-Perard, C., Lamy, B., Aldebert, D., Lemaire-Vieille, C., Grillot, R., Brion, J.-P., Gagnon, J., Cesbron, J.-Y. (2008). Aspergillus Conidia Activate the Complement by the Mannan-Binding Lectin C2 Bypass Mechanism. J. Immunol. 181: 7100-7105 [Abstract] [Full Text]  
  • Ramasawmy, R., Spina, G. S., Fae, K. C., Pereira, A. C., Nisihara, R., Messias Reason, I. J., Grinberg, M., Tarasoutchi, F., Kalil, J., Guilherme, L. (2008). Association of Mannose-Binding Lectin Gene Polymorphism but Not of Mannose-Binding Serine Protease 2 with Chronic Severe Aortic Regurgitation of Rheumatic Etiology. CVI 15: 932-936 [Abstract] [Full Text]  
  • Brouwer, N., Dolman, K. M., van Houdt, M., Sta, M., Roos, D., Kuijpers, T. W. (2008). Mannose-Binding Lectin (MBL) Facilitates Opsonophagocytosis of Yeasts but Not of Bacteria despite MBL Binding. J. Immunol. 180: 4124-4132 [Abstract] [Full Text]  
  • Koneti, A., Linke, M. J., Brummer, E., Stevens, D. A. (2008). Evasion of Innate Immune Responses: Evidence for Mannose Binding Lectin Inhibition of Tumor Necrosis Factor Alpha Production by Macrophages in Response to Blastomyces dermatitidis. Infect. Immun. 76: 994-1002 [Abstract] [Full Text]  
  • Aoyagi, Y., Adderson, E. E., Rubens, C. E., Bohnsack, J. F., Min, J. G., Matsushita, M., Fujita, T., Okuwaki, Y., Takahashi, S. (2008). L-Ficolin/Mannose-Binding Lectin-Associated Serine Protease Complexes Bind to Group B Streptococci Primarily through N-Acetylneuraminic Acid of Capsular Polysaccharide and Activate the Complement Pathway. Infect. Immun. 76: 179-188 [Abstract] [Full Text]  
  • van Till, J. W. O., Modderman, P. W., de Boer, M., Hart, M. H. L., Beld, M. G. H. M., Boermeester, M. A. (2008). Mannose-Binding Lectin Deficiency Facilitates Abdominal Candida Infections in Patients with Secondary Peritonitis. CVI 15: 65-70 [Abstract] [Full Text]  
  • Bulgakov, A. A, Eliseikina, M. G, Petrova, I. Y., Nazarenko, E. L, Kovalchuk, S. N, Kozhemyako, V. B, Rasskazov, V. A (2007). Molecular and Biological Characterization of a Mannan-Binding Lectin from the Holothurian Apostichopus Japonicus. Glycobiology 17: 1284-1298 [Abstract] [Full Text]  
  • Panepinto, J. C., Komperda, K. W., Hacham, M., Shin, S., Liu, X., Williamson, P. R. (2007). Binding of Serum Mannan Binding Lectin to a Cell Integrity-Defective Cryptococcus neoformans ccr4{Delta} Mutant. Infect. Immun. 75: 4769-4779 [Abstract] [Full Text]  
  • Sziller, I., Babula, O., Ujhazy, A., Nagy, B., Hupuczi, P., Papp, Z., Linhares, I.M., Ledger, W.J., Witkin, S.S. (2007). Chlamydia trachomatis infection, Fallopian tube damage and a mannose-binding lectin codon 54 gene polymorphism. Hum Reprod 22: 1861-1865 [Abstract] [Full Text]  
  • Sziller, I., Babula, O., Hupuczi, P., Nagy, B., Rigo, B., Szabo, G., Papp, Z., Linhares, I.M., Witkin, S.S. (2007). Mannose-binding lectin (MBL) codon 54 gene polymorphism protects against development of pre-eclampsia, HELLP syndrome and pre-eclampsia-associated intrauterine growth restriction. Mol Hum Reprod 13: 281-285 [Abstract] [Full Text]  
  • Smithson, A., Munoz, A., Suarez, B., Soto, S. M., Perello, R., Soriano, A., Martinez, J. A., Vila, J., Horcajada, J. P., Mensa, J., Lozano, F. (2007). Association between Mannose-Binding Lectin Deficiency and Septic Shock following Acute Pyelonephritis Due to Escherichia coli. CVI 14: 256-261 [Abstract] [Full Text]  
  • Iwaki, D., Kanno, K., Takahashi, M., Endo, Y., Lynch, N. J., Schwaeble, W. J., Matsushita, M., Okabe, M., Fujita, T. (2006). Small Mannose-Binding Lectin-Associated Protein Plays a Regulatory Role in the Lectin Complement Pathway. J. Immunol. 177: 8626-8632 [Abstract] [Full Text]  
  • Moller-Kristensen, M., Ip, W. K. E., Shi, L., Gowda, L. D., Hamblin, M. R., Thiel, S., Jensenius, J. Chr., Ezekowitz, R. A. B., Takahashi, K. (2006). Deficiency of Mannose-Binding Lectin Greatly Increases Susceptibility to Postburn Infection with Pseudomonas aeruginosa. J. Immunol. 176: 1769-1775 [Abstract] [Full Text]  
  • Lam, M. F., Leung, J. C. K., Tang, C. C. S., Lo, W. K., Tse, K. C., Yip, T. P., Lui, S. L., Chan, T. M., Lai, K. N. (2005). Mannose binding lectin level and polymorphism in patients on long-term peritoneal dialysis. Nephrol Dial Transplant 20: 2489-2496 [Abstract] [Full Text]  
  • Hamvas, R. M. J., Johnson, M., Vlieger, A. M., Ling, C., Sherriff, A., Wade, A., Klein, N. J., Turner, M. W., The ALSPAC Study Team, , Webster, A. D. B. (2005). Role for Mannose Binding Lectin in the Prevention of Mycoplasma Infection. Infect. Immun. 73: 5238-5240 [Abstract] [Full Text]  
  • Nadesalingam, J., Dodds, A. W., Reid, K. B. M., Palaniyar, N. (2005). Mannose-Binding Lectin Recognizes Peptidoglycan via the N-Acetyl Glucosamine Moiety, and Inhibits Ligand-Induced Proinflammatory Effect and Promotes Chemokine Production by Macrophages. J. Immunol. 175: 1785-1794 [Abstract] [Full Text]  
  • Teillet, F., Dublet, B., Andrieu, J.-P., Gaboriaud, C., Arlaud, G. J., Thielens, N. M. (2005). The Two Major Oligomeric Forms of Human Mannan-Binding Lectin: Chemical Characterization, Carbohydrate-Binding Properties, and Interaction with MBL-Associated Serine Proteases. J. Immunol. 174: 2870-2877 [Abstract] [Full Text]  
  • Krarup, A., Sorensen, U. B. S., Matsushita, M., Jensenius, J. C., Thiel, S. (2005). Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin. Infect. Immun. 73: 1052-1060 [Abstract] [Full Text]  
  • Baelder, R., Fuchs, B., Bautsch, W., Zwirner, J., Kohl, J., Hoymann, H. G, Glaab, T., Erpenbeck, V., Krug, N., Braun, A. (2005). Pharmacological Targeting of Anaphylatoxin Receptors during the Effector Phase of Allergic Asthma Suppresses Airway Hyperresponsiveness and Airway Inflammation. J. Immunol. 174: 783-789 [Abstract] [Full Text]  
  • Aoyagi, Y., Adderson, E. E., Min, J. G., Matsushita, M., Fujita, T., Takahashi, S., Okuwaki, Y., Bohnsack, J. F. (2005). Role of L-Ficolin/Mannose-Binding Lectin-Associated Serine Protease Complexes in the Opsonophagocytosis of Type III Group B Streptococci. J. Immunol. 174: 418-425 [Abstract] [Full Text]  
  • Ma, Y. G., Cho, M. Y., Zhao, M., Park, J. W., Matsushita, M., Fujita, T., Lee, B. L. (2004). Human Mannose-binding Lectin and L-Ficolin Function as Specific Pattern Recognition Proteins in the Lectin Activation Pathway of Complement. J. Biol. Chem. 279: 25307-25312 [Abstract] [Full Text]  
  • Dahl, M., Tybjaerg-Hansen, A., Schnohr, P., Nordestgaard, B. G. (2004). A Population-based Study of Morbidity and Mortality in Mannose-binding Lectin Deficiency. JEM 199: 1391-1399 [Abstract] [Full Text]  
  • Larsen, F., Madsen, H. O., Sim, R. B., Koch, C., Garred, P. (2004). Disease-associated Mutations in Human Mannose-binding Lectin Compromise Oligomerization and Activity of the Final Protein. J. Biol. Chem. 279: 21302-21311 [Abstract] [Full Text]  
  • Ferguson, J. S., Weis, J. J., Martin, J. L., Schlesinger, L. S. (2004). Complement Protein C3 Binding to Mycobacterium tuberculosis Is Initiated by the Classical Pathway in Human Bronchoalveolar Lavage Fluid. Infect. Immun. 72: 2564-2573 [Abstract] [Full Text]  
  • Mueller-Ortiz, S. L., Drouin, S. M., Wetsel, R. A. (2004). The Alternative Activation Pathway and Complement Component C3 Are Critical for a Protective Immune Response against Pseudomonas aeruginosa in a Murine Model of Pneumonia. Infect. Immun. 72: 2899-2906 [Abstract] [Full Text]  
  • Hogaboam, C. M., Takahashi, K., Ezekowitz, R. A. B., Kunkel, S. L., Schuh, J. M. (2004). Mannose-binding lectin deficiency alters the development of fungal asthma: effects on airway response, inflammation, and cytokine profile. J. Leukoc. Biol. 75: 805-814 [Abstract] [Full Text]  
  • Estabrook, M. M., Jack, D. L., Klein, N. J., Jarvis, G. A. (2004). Mannose-Binding Lectin Binds to Two Major Outer Membrane Proteins, Opacity Protein and Porin, of Neisseria meningitidis. J. Immunol. 172: 3784-3792 [Abstract] [Full Text]  
  • de Haas, C. J.C., Veldkamp, K. E., Peschel, A., Weerkamp, F., Van Wamel, W. J.B., Heezius, E. C.J.M., Poppelier, M. J.J.G., Van Kessel, K. P.M., van Strijp, J. A.G. (2004). Chemotaxis Inhibitory Protein of Staphylococcus aureus, a Bacterial Antiinflammatory Agent. JEM 199: 687-695 [Abstract] [Full Text]  
  • Lynch, N. J., Roscher, S., Hartung, T., Morath, S., Matsushita, M., Maennel, D. N., Kuraya, M., Fujita, T., Schwaeble, W. J. (2004). L-Ficolin Specifically Binds to Lipoteichoic Acid, a Cell Wall Constituent of Gram-Positive Bacteria, and Activates the Lectin Pathway of Complement. J. Immunol. 172: 1198-1202 [Abstract] [Full Text]  
  • Romani, L., Montagnoli, C., Bozza, S., Perruccio, K., Spreca, A., Allavena, P., Verbeek, S., Calderone, R. A., Bistoni, F., Puccetti, P. (2004). The exploitation of distinct recognition receptors in dendritic cells determines the full range of host immune relationships with Candida albicans. Int Immunol 16: 149-161 [Abstract] [Full Text]  
  • Carlsson, F., Berggard, K., Stalhammar-Carlemalm, M., Lindahl, G. (2003). Evasion of Phagocytosis through Cooperation between Two Ligand-binding Regions in Streptococcus pyogenes M Protein. JEM 198: 1057-1068 [Abstract] [Full Text]  
  • Kvalsvig, A J, Unsworth, D J (2003). The immunopathogenesis of meningococcal disease. J. Clin. Pathol. 56: 417-422 [Abstract] [Full Text]  
  • Rogers, P. D., Thornton, J., Barker, K. S., McDaniel, D. O., Sacks, G. S., Swiatlo, E., McDaniel, L. S. (2003). Pneumolysin-Dependent and -Independent Gene Expression Identified by cDNA Microarray Analysis of THP-1 Human Mononuclear Cells Stimulated by Streptococcus pneumoniae. Infect. Immun. 71: 2087-2094 [Abstract] [Full Text]  
  • Hart, M. L., Saifuddin, M., Spear, G. T. (2003). Glycosylation inhibitors and neuraminidase enhance human immunodeficiency virus type 1 binding and neutralization by mannose-binding lectin. J. Gen. Virol. 84: 353-360 [Abstract] [Full Text]  
  • Brown, J. S., Hussell, T., Gilliland, S. M., Holden, D. W., Paton, J. C., Ehrenstein, M. R., Walport, M. J., Botto, M. (2002). The classical pathway is the dominant complement pathway required for innate immunity to Streptococcus pneumoniae infection in mice. Proc. Natl. Acad. Sci. USA 99: 16969-16974 [Abstract] [Full Text]  
  • Lee, S. J., Gonzalez-Aseguinolaza, G., Nussenzweig, M. C. (2002). Disseminated Candidiasis and Hepatic Malarial Infection in Mannose-Binding-Lectin-A-Deficient Mice. Mol. Cell. Biol. 22: 8199-8203 [Abstract] [Full Text]  
  • Hartshorn, K. L., White, M. R., Crouch, E. C. (2002). Contributions of the N- and C-Terminal Domains of Surfactant Protein D to the Binding, Aggregation, and Phagocytic Uptake of Bacteria. Infect. Immun. 70: 6129-6139 [Abstract] [Full Text]  
  • Zamze, S., Martinez-Pomares, L., Jones, H., Taylor, P. R., Stillion, R. J., Gordon, S., Wong, S. Y. C. (2002). Recognition of Bacterial Capsular Polysaccharides and Lipopolysaccharides by the Macrophage Mannose Receptor. J. Biol. Chem. 277: 41613-41623 [Abstract] [Full Text]  
  • Neth, O., Jack, D. L., Johnson, M., Klein, N. J., Turner, M. W. (2002). Enhancement of Complement Activation and Opsonophagocytosis by Complexes of Mannose-Binding Lectin with Mannose-Binding Lectin-Associated Serine Protease After Binding to Staphylococcus aureus. J. Immunol. 169: 4430-4436 [Abstract] [Full Text]  
  • Scanlan, C. N., Pantophlet, R., Wormald, M. R., Ollmann Saphire, E., Stanfield, R., Wilson, I. A., Katinger, H., Dwek, R. A., Rudd, P. M., Burton, D. R. (2002). The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of {alpha}1->2 Mannose Residues on the Outer Face of gp120. J. Virol. 76: 7306-7321 [Abstract] [Full Text]  
  • Mullighan, C. G., Heatley, S., Doherty, K., Szabo, F., Grigg, A., Hughes, T. P., Schwarer, A. P., Szer, J., Tait, B. D., Bik To, L., Bardy, P. G. (2002). Mannose-binding lectin gene polymorphisms are associated with major infection following allogeneic hemopoietic stem cell transplantation. Blood 99: 3524-3529 [Abstract] [Full Text]  
  • van Crevel, R., Ottenhoff, T. H. M., van der Meer, J. W. M. (2002). Innate Immunity to Mycobacterium tuberculosis. Clin. Microbiol. Rev. 15: 294-309 [Abstract] [Full Text]  
  • Wilson, J W, Schurr, M J, LeBlanc, C L, Ramamurthy, R, Buchanan, K L, Nickerson, C A (2002). Mechanisms of bacterial pathogenicity. Postgrad. Med. J. 78: 216-224 [Abstract] [Full Text]  
  • Marr, K. A., Koudadoust, M., Black, M., Balajee, S. A. (2001). Early Events in Macrophage Killing of Aspergillus fumigatus Conidia: New Flow Cytometric Viability Assay. CVI 8: 1240-1247 [Abstract] [Full Text]  
  • Hansen, T. K., Thiel, S., Dall, R., Rosenfalck, A. M., Trainer, P., Flyvbjerg, A., Jorgensen, J. O. L., Christiansen, J. S. (2001). GH Strongly Affects Serum Concentrations of Mannan-Binding Lectin: Evidence for a New IGF-I Independent Immunomodulatory Effect of GH. J. Clin. Endocrinol. Metab. 86: 5383-5388 [Abstract] [Full Text]  
  • Roos, A., Bouwman, L. H., van Gijlswijk-Janssen, D. J., Faber-Krol, M. C., Stahl, G. L., Daha, M. R. (2001). Human IgA Activates the Complement System Via the Mannan-Binding Lectin Pathway. J. Immunol. 167: 2861-2868 [Abstract] [Full Text]  
  • Devyatyarova-Johnson, M., Rees, I. H., Robertson, B. D., Turner, M. W., Klein, N. J., Jack, D. L. (2000). The Lipopolysaccharide Structures of Salmonella enterica Serovar Typhimurium and Neisseria gonorrhoeae Determine the Attachment of Human Mannose-Binding Lectin to Intact Organisms. Infect. Immun. 68: 3894-3899 [Abstract] [Full Text]  
  • Turner, M. W., Dinan, L., Heatley, S., Jack, D. L., Boettcher, B., Lester, S., McCluskey, J., Roberton, D. (2000). Restricted polymorphism of the mannose-binding lectin gene of indigenous Australians. Hum Mol Genet 9: 1481-1486 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»