This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haller, D.
Right arrow Articles by Schiffrin, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haller, D.
Right arrow Articles by Schiffrin, E. J.

 Previous Article  |  Next Article 

Infection and Immunity, February 2000, p. 752-759, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Activation of Human Peripheral Blood Mononuclear Cells by Nonpathogenic Bacteria In Vitro: Evidence of NK Cells as Primary Targets

D. Haller,1,2,* S. Blum,3 C. Bode,1 W. P. Hammes,2 and E. J. Schiffrin3

Institute of Biological Chemistry and Nutrition Science1 and Institute of Food Technology,2 University Hohenheim, D-70599 Stuttgart, Germany, and Department of Immunology, Nestlé Research Centre, 1000 Lausanne 26, Switzerland3

Received 23 August 1999/Returned for modification 7 October 1999/Accepted 4 November 1999

The interaction of commensal bacteria with immunocompetent cells may occur in definite compartments of the mucosal immune system, as limited translocation through the epithelial barrier cannot be excluded. In this study the stimulation of human peripheral blood mononuclear cells and purified lymphocyte subsets by nonpathogenic gram-positive lactobacilli (Lactobacillus johnsonii and Lactobacillus sakei) and gram-negative Escherichia coli was investigated. The various bacterial strains induced a differential cytokine pattern. Whereas L. johnsonii and L. sakei strongly induced gamma interferon (IFN-gamma ) and interleukin-12 (IL-12), E. coli and lipopolysaccharide (LPS) preferentially induced IL-10 after 16 h of stimulation. Expression of activation antigens CD69 and CD25 was observed on (CD3- CD56+) natural killer (NK) cells after stimulation of total human peripheral blood mononuclear cells. All bacteria mediated the proliferation of human peripheral blood mononuclear cells, and the strongest proliferative response was observed with L. johnsonii. Purified CD4+, CD8+, and CD19+ lymphocyte subsets were not activated upon bacterial stimulation but showed normal response to a mitogenic stimulus. In contrast, purified NK cells upregulated the IL-2Ralpha chain (CD25) and underwent proliferation when stimulated by L. johnsonii. E. coli and LPS were less effective in inducing proliferation. Expression of CD25 or secretion of IFN-gamma from purified NK cells was significantly increased in the presence of bacterially primed macrophages, indicating that full activation required both bacterium- and cell contact-based signals derived from accessory cells.

* Corresponding author. Mailing address: Dept. of Immunology, Nestlé Research Centre, Vers-Chez-les-Blanc, 1000 Lausanne 26, Switzerland. Phone: 41-21-785-8513. Fax: 41-21-785-8925. E-mail: dirk.haller{at}rdls.nestle.com.

Infection and Immunity, February 2000, p. 752-759, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fleige, S., Preissinger, W., Meyer, H. H. D., Pfaffl, M. W. (2009). The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves. J ANIM SCI 87: 1731-1738 [Abstract] [Full Text]  
  • Fink, L. N., Zeuthen, L. H., Christensen, H. R., Morandi, B., Frokiaer, H., Ferlazzo, G. (2007). Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses. Int Immunol 19: 1319-1327 [Abstract] [Full Text]  
  • Shida, K., Suzuki, T., Kiyoshima-Shibata, J., Shimada, S.-i., Nanno, M. (2006). Essential Roles of Monocytes in Stimulating Human Peripheral Blood Mononuclear Cells with Lactobacillus casei To Produce Cytokines and Augment Natural Killer Cell Activity.. CVI 13: 997-1003 [Abstract] [Full Text]  
  • Benyacoub, J., Perez, P. F., Rochat, F., Saudan, K. Y., Reuteler, G., Antille, N., Humen, M., De Antoni, G. L., Cavadini, C., Blum, S., Schiffrin, E. J. (2005). Enterococcus faecium SF68 Enhances the Immune Response to Giardia intestinalis in Mice. J. Nutr. 135: 1171-1176 [Abstract] [Full Text]  
  • Humen, M. A., De Antoni, G. L., Benyacoub, J., Costas, M. E., Cardozo, M. I., Kozubsky, L., Saudan, K.-Y., Boenzli-Bruand, A., Blum, S., Schiffrin, E. J., Perez, P. F. (2005). Lactobacillus johnsonii La1 Antagonizes Giardia intestinalis In Vivo. Infect. Immun. 73: 1265-1269 [Abstract] [Full Text]  
  • Boekhorst, J., Siezen, R. J., Zwahlen, M.-C., Vilanova, D., Pridmore, R. D., Mercenier, A., Kleerebezem, M., de Vos, W. M., Brussow, H., Desiere, F. (2004). The complete genomes of Lactobacillus plantarum and Lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content. Microbiology 150: 3601-3611 [Abstract] [Full Text]  
  • Tannock, G. W. (2004). A Special Fondness for Lactobacilli. Appl. Environ. Microbiol. 70: 3189-3194 [Full Text]  
  • Pridmore, R. D., Berger, B., Desiere, F., Vilanova, D., Barretto, C., Pittet, A.-C., Zwahlen, M.-C., Rouvet, M., Altermann, E., Barrangou, R., Mollet, B., Mercenier, A., Klaenhammer, T., Arigoni, F., Schell, M. A. (2004). The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533. Proc. Natl. Acad. Sci. USA 101: 2512-2517 [Abstract] [Full Text]  
  • Benyacoub, J., Czarnecki-Maulden, G. L., Cavadini, C., Sauthier, T., Anderson, R. E., Schiffrin, E. J., von der Weid, T. (2003). Supplementation of Food with Enterococcus faecium (SF68) Stimulates Immune Functions in Young Dogs. J. Nutr. 133: 1158-1162 [Abstract] [Full Text]  
  • Ibnou-Zekri, N., Blum, S., Schiffrin, E. J., von der Weid, T. (2003). Divergent Patterns of Colonization and Immune Response Elicited from Two Intestinal Lactobacillus Strains That Display Similar Properties In Vitro. Infect. Immun. 71: 428-436 [Abstract] [Full Text]  
  • Karlsson, H., Hessle, C., Rudin, A. (2002). Innate Immune Responses of Human Neonatal Cells to Bacteria from the Normal Gastrointestinal Flora. Infect. Immun. 70: 6688-6696 [Abstract] [Full Text]  
  • Haller, D., Serrant, P., Granato, D., Schiffrin, E. J., Blum, S. (2002). Activation of Human NK Cells by Staphylococci and Lactobacilli Requires Cell Contact-Dependent Costimulation by Autologous Monocytes. CVI 9: 649-657 [Abstract] [Full Text]  
  • Robinson, K., Neal, K. R., Howard, C., Stockton, J., Atkinson, K., Scarth, E., Moran, J., Robins, A., Todd, I., Kaczmarski, E., Gray, S., Muscat, I., Slack, R., Ala'Aldeen, D. A. A. (2002). Characterization of Humoral and Cellular Immune Responses Elicited by Meningococcal Carriage. Infect. Immun. 70: 1301-1309 [Abstract] [Full Text]  
  • Tsirpouchtsidis, A., Hurwitz, R., Brinkmann, V., Meyer, T. F., Haas, G. (2002). Neisserial Immunoglobulin A1 Protease Induces Specific T-Cell Responses in Humans. Infect. Immun. 70: 335-344 [Abstract] [Full Text]  
  • von der Weid, T., Bulliard, C., Schiffrin, E. J. (2001). Induction by a Lactic Acid Bacterium of a Population of CD4+ T Cells with Low Proliferative Capacity That Produce Transforming Growth Factor {beta} and Interleukin-10. CVI 8: 695-701 [Abstract] [Full Text]  
  • Neeser, J.-R., Granato, D., Rouvet, M., Servin, A., Teneberg, S., Karlsson, K.-A. (2000). Lactobacillus johnsonii La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria. Glycobiology 10: 1193-1199 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»