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Infection and Immunity, February 2000, p. 760-766, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunogenicity of the B Monomer of Escherichia coli Heat-Labile Toxin Expressed on the Surface of Streptococcus gordonii

Susanna Ricci,1,dagger Donata Medaglini,1 Catherine M. Rush,1,Dagger Alessandro Marcello,2,§ Samuele Peppoloni,3 Riccardo Manganelli,1,par Giorgio Palú,2 and Gianni Pozzi1,*

Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena,1 Istituto di Microbiologia, Università di Padova, 35121 Padova,2 and IRIS Research Center, Chiron Vaccines, Siena,3 Italy

Received 7 September 1999/Accepted 17 November 1999

The B monomer of the Escherichia coli heat-labile toxin (LTB) was expressed on the surface of the human oral commensal bacterium Streptococcus gordonii. Recombinant bacteria expressing LTB were used to immunize BALB/c mice subcutaneously and intragastrically. The LTB monomer expressed on the streptococcal surface proved to be highly immunogenic, as LTB-specific immunoglobulin G (IgG) serum titers of 140,000 were induced after systemic immunization. Most significantly, these antibodies were capable of neutralizing the enterotoxin in a cell neutralization assay. Following mucosal delivery, antigen-specific IgA antibodies were found in feces and antigen-specific IgG antibodies were found in sera. Analysis of serum IgG subclasses showed a clear predominance of IgG1 when recombinant bacteria were inoculated subcutaneously, while a prevalence of IgG2a was observed upon intragastric delivery, suggesting, in this case, the recruitment of a Th1 type of immune response.

* Corresponding author. Mailing address: Microbiologia, Università di Siena, Via Laterina 8, 53100 Siena, Italy. Phone: 39-577-233874. Fax: 39-577-233870. E-mail: pozzi{at}unisi.it.

dagger Present address: Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, S-221 00 Lund, Sweden.

Dagger Present address: Department of Pathology, Section of Microbiology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.

§ Present address: International Centre for Genetic Engineering and Biotechnology (ICGEB), Laboratory of Molecular Biology, AREA Science Park, I-34012 Trieste, Italy.

par Present address: Department of Microbiology, Public Health Research Institute, New York, NY 10016.

Infection and Immunity, February 2000, p. 760-766, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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