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Infection and Immunity, February 2000, p. 796-800, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Intranasal Immunization of Mice with a Mixture of the Pneumococcal Proteins PsaA and PspA Is Highly Protective against Nasopharyngeal Carriage of Streptococcus pneumoniae

David E. Briles,1,2,* Eddie Ades,3 James C. Paton,4 Jacquelyn S. Sampson,3 George M. Carlone,3 Robert C. Huebner,5 Anni Virolainen,1,6 Edwin Swiatlo,1,7 and Susan K. Hollingshead1

Department of Microbiology1 and Department of Pediatrics,2 University of Alabama at Birmingham, Birmingham, Alabama; Centers for Disease Control and Prevention, Atlanta, Georgia3; Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, Australia4; Pasteur Mérieux Connaught, Swiftwater, Pennsylvania5; Haartman Institute, Department of Bacteriology and Immunology, University of Helsinki, Finland6; and Division of Infectious Diseases, University of Mississippi Medical Center, Jackson, Mississippi7

Received 20 October 1999/Returned for modification 12 November 1999/Accepted 17 November 1999

Acquisition of pneumococci is generally from carriers rather than from infected individuals. Therefore, to induce herd immunity against Streptococcus pneumoniae it will be necessary to elicit protection against carriage. Capsular polysaccharide-protein conjugates, PspA, and PsaA are known to elicit some protection against nasopharyngeal carriage of pneumococci but do not always completely eliminate carriage. In this study, we observed that PsaA elicited better protection than did PspA against carriage. Pneumolysin elicited no protection against carriage. Immunization with a mixture of PsaA and PspA elicited the best protection against carriage. These results indicate that PspA and PsaA may be useful for the elicitation of herd immunity in humans. As PspA and pneumolysin are known to elicit immunity to bacteremia and pneumonia, their inclusion in a mucosal vaccine may enable such a vaccine to prevent invasive disease as well as carriage.

* Corresponding author. Mailing address: Department of Microbiology, UAB,658 Bevill Building, 845 19th St. South, Birmingham, AL 35294. Phone: (205) 934-6595. Fax: (205) 934-0605. E-mail: dbriles{at}uab.edu.

Infection and Immunity, February 2000, p. 796-800, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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