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Infection and Immunity, February 2000, p. 815-823, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Analysis of a Gene Cluster of Enterococcus
faecalis Involved in Polysaccharide Biosynthesis
Yi
Xu,1,2,
Kavindra V.
Singh,2,3
Xiang
Qin,2,3
Barbara E.
Murray,2,3 and
George M.
Weinstock1,2,*
Department of Microbiology and Molecular
Genetics,1 Division of Infectious
Diseases, Department of Medicine,3 and
Center for the Study of Emerging and Re-emerging
Pathogens,2 University of Texas Medical
School, Houston, Texas 77030
Received 22 July 1999/Returned for modification 8 September
1999/Accepted 9 November 1999
Previously, we described a gene cluster of Enterococcus
faecalis OG1RF that produced an antigenic polysaccharide when
cloned in Escherichia coli. The polysaccharide antigen was
not detectable in E. faecalis strains, however. Here, we
show by reverse transcriptase-PCR that the 16 genes in this region are
transcribed in OG1RF. Gene disruption of orfde4, encoding a
putative glycosyl transferase, and orfde6, a putative
dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The
mutants showed delayed killing and a higher 50% lethal dose in a mouse
peritonitis model. In addition, two mucoid E. faecalis
isolates from patients with chronic urinary tract infections were found
to produce the polysaccharide antigen.
*
Corresponding author. Mailing address: Department of
Microbiology and Molecular Genetics, University of Texas Medical
School, 6431 Fannin St., Houston, TX 77030. Phone: (713) 500-6083. Fax: (713) 500-5499. E-mail:
georgew{at}utmmg.med.uth.tmc.edu.

Present address: Center for Extracellular Matrix Biology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M
University,
Houston, TX 77030-3303.
Infection and Immunity, February 2000, p. 815-823, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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