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Infection and Immunity, February 2000, p. 925-930, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of the Operon for the Sorbitol (Glucitol) Phosphoenolpyruvate:Sugar Phosphotransferase System in Streptococcus mutans

David A. Boyd,1,dagger Tracy Thevenot,1,Dagger Markus Gumbmann,1 Allen L. Honeyman,2 and Ian R. Hamilton1,*

Department of Oral Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W2,1 and Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 336122

Received 24 June 1999/Returned for modification 17 August 1999/Accepted 4 October 1999

Transposon mutagenesis and marker rescue were used to isolate and identify an 8.5-kb contiguous region containing six open reading frames constituting the operon for the sorbitol P-enolpyruvate phosphotransferase transport system (PTS) of Streptococcus mutans LT11. The first gene, srlD, codes for sorbitol-6-phosphate dehydrogenase, followed downstream by srlR, coding for a transcriptional regulator; srlM, coding for a putative activator; and the srlA, srlE, and srlB genes, coding for the EIIC, EIIBC, and EIIA components of the sorbitol PTS, respectively. Among all sorbitol PTS operons characterized to date, the srlD gene is found after the genes coding for the EII components; thus, the location of the gene in S. mutans is unique. The SrlR protein is similar to several transcriptional regulators found in Bacillus spp. that contain PTS regulator domains (J. Stülke, M. Arnaud, G. Rapoport, and I. Martin-Verstraete, Mol. Microbiol. 28:865-874, 1998), and its gene overlaps the srlM gene by 1 bp. The arrangement of these two regulatory genes is unique, having not been reported for other bacteria.

* Corresponding author. Mailing address: Department of Oral Biology, University of Manitoba, 780 Bannatyne Ave., Winnipeg, Manitoba, Canada R3E 0W2. Phone: (204) 789-3615. Fax: (204) 789-3948. E-mail: ihamilt{at}cc.umanitoba.ca.

dagger Present address: Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada R3E 3P6.

Dagger Present address: Department of Veterinary Biosciences, Ohio State University, Columbus, OH 43210.

Infection and Immunity, February 2000, p. 925-930, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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