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Infection and Immunity, February 2000, p. 942-947, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Construction of Urease-Negative Mutants of Yersinia enterocolitica Serotypes O:3 and O:8: Role of Urease in Virulence and Arthritogenicity

Christel Gripenberg-Lerche,1,2,3,4,* Lijuan Zhang,1,2,5,dagger Päivi Ahtonen,1,2,5 Paavo Toivanen,1,2 and Mikael Skurnik5,6

Turku Immunology Center1 and Departments of Medical Microbiology,2 Medical Biochemistry,6 and Medicine,4 Turku University, National Public Health Institute,3 Turku Center for Biotechnology, Turku University, and Åbo Academy University,5 Turku, Finland

Received 27 July 1999/Returned for modification 5 October 1999/Accepted 8 November 1999

Yersinia enterocolitica serotype O:3 and O:8 urease-negative mutants unable to express the 19-kDa beta  subunit of urease were constructed and tested for virulence and arthritogenicity. Our results indicate that urease is needed for full virulence in oral infections and that it is not an arthritogenic factor in the rat model.

* Corresponding author. Mailing address: National Public Health Institute, Department in Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland. Phone: 358-2-2519255. Fax: 358-2-2519254. E-mail: griler{at}utu.fi.

dagger Present address: Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada.

Infection and Immunity, February 2000, p. 942-947, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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