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Infection and Immunity, February 2000, p. 969-972, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Intranasal Immunization with Toxoplasma gondii SAG1 Induces Protective Cells into Both NALT and GALT Compartments

F. Velge-Roussel,* P. Marcelo, A. C. Lepage, D. Buzoni-Gatel, and D. T. Bout

Equipe Associée INRA d'Immunologie Parasitaire-EA 2637, UFR des Sciences Pharmaceutiques, 37200 Tours, France

Received 21 July 1999/Returned for modification 20 October 1999/Accepted 10 November 1999

Intranasal (i.n.) immunization with the SAG1 protein of Toxoplasma gondii plus cholera toxin (CT) provides protective immunity. The aim of this study was to analyze the cellular activation of several mucosal compartments after i.n. immunization. Cervical and mesenteric lymph node (CLN and MLN, respectively) lymphoid cell and intraepithelial lymphocyte (IEL) passive transfer experiments were performed with CBA/J mice immunized i.n. with SAG1 plus CT. CLN and MLN cells and IEL isolated 42 days after immunization conferred protective immunity on naive recipient mice challenged with strain 76K T. gondii, as assessed by the reduction in the number of brain cysts. There were proliferative specific responses in nose-associated lymphoid tissue and the CLN and MLN cells from mice immunized with SAG1 plus CT, but no cytokine was detectable. Thus, protective immunity is associated with a specific cellular response in the nasal and mesenteric compartments after i.n. immunization.

* Corresponding author. Mailing address: Equipe Associée INRA d'Immunologie Parasitaire-EA 2637, UFR des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France. Phone: 33 02 47 36 71 86. Fax: 33 02 47 36 72 52. E-mail: velge{at}univ-tours.fr.

Infection and Immunity, February 2000, p. 969-972, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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