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Infection and Immunity, February 2000, p. 982-985, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of the Roles of Calcineurin in Physiology and Virulence in Serotype D and Serotype A Strains of Cryptococcus neoformans

M. Cristina Cruz,1 Rey A. L. Sia,1 Michael Olson,2 Gary M. Cox,3 and Joseph Heitman1,2,3,4,5,*

Departments of Genetics,1 Pharmacology and Cancer Biology,2 Microbiology,4 and Medicine,3 and the Howard Hughes Medical Institute,5 Duke University Medical Center, Durham, North Carolina 27710

Received 1 July 1999/Returned for modification 3 September 1999/Accepted 7 November 1999

The calcineurin gene was cloned and disrupted in serotype D strains of Cryptococcus neoformans. Serotype A and serotype D calcineurin mutants were inviable at 37°C and avirulent in mice, whereas only serotype A mutants were cation stress sensitive. Thus, calcineurin plays conserved and divergent roles in serotype A and serotype D strains.

* Corresponding author. Mailing address: Box 3546, 322 CARL Bldg., Research Dr., Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-2824. Fax: (919) 684-5458. E-mail: heitm001{at}mc.duke.edu.

Infection and Immunity, February 2000, p. 982-985, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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