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Infection and Immunity, February 2000, p. 990-993, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

MTSA-10, the Product of the Rv3874 Gene of Mycobacterium tuberculosis, Elicits Tuberculosis-Specific, Delayed-Type Hypersensitivity in Guinea Pigs

Roberto Colangeli,1 John S. Spencer,2 Pablo Bifani,1 Alan Williams,3 Konstantin Lyashchenko,1 Marc A. Keen,2 Preston J. Hill,2 John Belisle,2 and Maria Laura Gennaro1,*

Public Health Research Institute, New York, New York 100161; Colorado State University, Fort Collins, Colorado 805232; and Amersham-Pharmacia Biotech, Piscataway, New Jersey 08855-13273

Received 12 July 1999/Returned for modification 24 August 1999/Accepted 21 October 1999

In a search for new skin test reagents specific for tuberculosis, we found that the antigen encoded by gene Rv3874 of Mycobacterium tuberculosis elicited delayed-type hypersensitivity in M. tuberculosis-infected guinea pigs but not in control animals immunized with Mycobacterium bovis bacillus Calmette-Guérin (BCG) or Mycobacterium avium. The antigen, which was named MTSA-10 (for M. tuberculosis-specific antigen 10), is a prime candidate for a component of a new tuberculin that will allow discrimination by a skin test of latent M. tuberculosis infection from vaccination with BCG or from sensitization with environmental, nontuberculous mycobacteria.

* Corresponding author. Mailing address: Public Health Research Institute, 455 First Ave., New York, NY 10016. Phone: (212) 578-0844. Fax: (212) 578-0804. E-mail: gennaro{at}phri.nyu.edu.

Infection and Immunity, February 2000, p. 990-993, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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