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Infection and Immunity, March 2000, p. 1080-1085, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Eukaryotic Cell Uptake of Heparin-Coated Microspheres: a Model of Host Cell Invasion by Chlamydia trachomatis

Richard S. Stephens,1,2,3,* Farah S. Fawaz,4 Kathleen A. Kennedy,2 Kelly Koshiyama,1 Barbara Nichols,3 Christiaan van Ooij,5 and Joanne N. Engel4

Program in Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720,1 and Departments of Laboratory Medicine,2 Medicine,4 and Biomedical Sciences5 and the Francis I. Proctor Foundation,3 University of California, San Francisco, California 94143

Received 12 July 1999/Returned for modification 30 August 1999/Accepted 15 November 1999

Using polystyrene microspheres coated with heparin or heparan sulfate, it was shown that coated microspheres specifically bound eukaryotic cells and were endocytosed by nonprofessional phagocytic cells. Coated microspheres displayed properties of binding to eukaryotic cells that were similar to those of chlamydiae, and the microspheres were competitively inhibited by chlamydial organisms. Endocytosis of heparin-coated beads resulted in the tyrosine phosphorylation of a similar set of host proteins as did endocytosis of chlamydiae; however, unlike viable chlamydial organisms, which prevent phagolysosomal fusion, endocytosed beads were trafficked to a lysosomal compartment. These findings suggest that heparin-coated beads and Chlamydia trachomatis enter eukaryotic cells by similar pathways.

* Corresponding author. Mailing address: Program in Infectious Diseases, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360. Phone: (510) 643-9900. Fax: (510) 643-5676. E-mail: rss{at}uclink4.berkeley.edu.

Infection and Immunity, March 2000, p. 1080-1085, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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