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Infection and Immunity, March 2000, p. 1282-1288, Vol. 68, No. 3
0019-9567/00/$04.00+0

Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes

Cheol H. Yun,dagger Hyun S. Lillehoj,* and Kang D. Choi

Immunology and Disease Resistance Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland 20705

Received 23 September 1999/Returned for modification 1 November 1999/Accepted 8 December 1999

The role of intestinal lymphocytes and gamma interferon (IFN-gamma ) production in protective immunity to Eimeria tenella infection was evaluated in two inbred strains of chickens (SC and TK) that display different patterns of susceptibility to coccidiosis. Oral inoculation of either strain with E. tenella led to parasite invasion of the intestinal cecum and cecal tonsils. Greater fecal oocyst shedding was seen in TK chickens. Flow cytometric analyses of cecal tonsil lymphocytes demonstrated greater numbers of CD4+ and T-cell receptor gamma delta -positive (TCR1+) cells in SC chickens and elevated numbers of CD8+ and TCR2+ cells in TK chickens following primary infection. IFN-gamma mRNA expression was significantly increased in cecal tonsil and intraepithelial lymphocytes at days 6 and 8, respectively, after primary infection in SC compared to TK chickens. While no differences were noted between cecal tonsil lymphocytes of the two strains following secondary infection, TK chickens showed elevated IFN-gamma transcript levels in intestinal intraepithelial lymphocytes at this time. Selective depletion of CD4+, but not CD8+, cecal tonsil lymphocytes in SC chickens resulted in a reduced IFN-gamma mRNA expression, indicating that CD4+ cells are the primary source of this cytokine. Collectively, these results indicate that local lymphocyte responses and production of IFN-gamma are influenced by host genetic factors.

* Corresponding author. Mailing address: BARC-East, Building 1040, IDRL, LPSI, USDA-ARS, Beltsville, MD 20705. Phone: (301) 504-8771. Fax: (301) 504-5306. E-mail: hlilleho{at}lpsi.barc.usda.gov.

dagger Present address: Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255.

Infection and Immunity, March 2000, p. 1282-1288, Vol. 68, No. 3
0019-9567/00/$04.00+0


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