Previous Article | Next Article 
Infection and Immunity, March 2000, p. 1474-1479, Vol. 68, No. 3
Department of
Medicine/Infectious Diseases, University of Florida, Gainesville,
Florida 32610,1 and Department of
Microbiology, University of Washington, Seattle, Washington
981452
Received Received 15 October 1999/Returned for modification 17 November
1999/Accepted 7 December 1999
Binding of Pseudomonas aeruginosa strain PAK to mucin
has been shown to be mediated by the flagellar cap protein, product of
the fliD gene. Since the flagellar cap is very likely an
exposed structure, the FliD polypeptide should be recognized by the
host immune system, analogous to the recognition of dominant epitopes located in the exposed parts of the flagellin polypeptide within the
assembled flagellum. In P. aeruginosa, a number of distinct flagellin variants are made, and these variable sequences presumably allow the newly infected P. aeruginosa to escape
recognition by the antibody induced during a previous infection. Since
similar mechanisms may direct the selection of FliD variants, we
examined the extent of sequence heterogeneity among various FliD
sequences among a selected group of P. aeruginosa. The
results of PCR and nucleotide sequencing of the fliD region
of eight different P. aeruginosa strains (laboratory
strains PAK, PAO1, and PA103; clinical strains 1244, CS2, and CS32;
cystic fibrosis strains CS29 and MDR) suggested that there were two
distinct types of FliD in P. aeruginosa, which we named A
type and B type. The results of Western blotting using the polyclonal
antibodies raised against the purified FliD of A type (PAK) or B type
(PAO1) further confirmed the existence of two distinct antigenic types
of FliD proteins, with no cross-reactivity between the two serotypes.
Further Western immunoblot analysis of the same strains using
polyclonal FliC antibody showed that the strains with A-type FliD
possessed a-type FliC and those with B-type FliD had b-type FliC.
Similar Western blot analyses of 50 more P. aeruginosa
strains obtained from varied sources revealed that all strains
contained either A-type or B-type FliD, suggesting the existence of
only two types of FliD in P. aeruginosa and indicating that
fliC and fliD were coinherited. This limited
diversity of FliC and FliD serotypes seems to be a unique feature of
flagellar proteins. A chromosomal mutant having an insertion in the
fliD gene of P. aeruginosa PAO1 was
constructed. The motility defect of this mutant and a previously
constructed PAK fliD mutant was better complemented with
the fliD gene of the homologous types.
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Identification of Two Distinct Types
of Flagellar Cap Proteins, FliD, in Pseudomonas
aeruginosa
*
Corresponding author. Mailing address: Department of
Medicine/Infectious Diseases, P.O. Box 100277, JHMHC, University of
Florida, Gainesville, FL 32610. Phone: (352) 392-2932. Fax: (352)
392-6481. E-mail: RAMPHR{at}medmac.ufl.edu.
Infection and Immunity, March 2000, p. 1474-1479, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Holden, M. T. G., Seth-Smith, H. M. B., Crossman, L. C., Sebaihia, M., Bentley, S. D., Cerdeno-Tarraga, A. M., Thomson, N. R., Bason, N., Quail, M. A., Sharp, S., Cherevach, I., Churcher, C., Goodhead, I., Hauser, H., Holroyd, N., Mungall, K., Scott, P., Walker, D., White, B., Rose, H., Iversen, P., Mil-Homens, D., Rocha, E. P. C., Fialho, A. M., Baldwin, A., Dowson, C., Barrell, B. G., Govan, J. R., Vandamme, P., Hart, C. A., Mahenthiralingam, E., Parkhill, J.
(2009). The Genome of Burkholderia cenocepacia J2315, an Epidemic Pathogen of Cystic Fibrosis Patients. J. Bacteriol.
191: 261-277
[Abstract] [Full Text] -
Correa, N. E., Peng, F., Klose, K. E.
(2005). Roles of the Regulatory Proteins FlhF and FlhG in the Vibrio cholerae Flagellar Transcription Hierarchy. J. Bacteriol.
187: 6324-6332
[Abstract] [Full Text] -
Capdevila, S., Martinez-Granero, F. M., Sanchez-Contreras, M., Rivilla, R., Martin, M.
(2004). Analysis of Pseudomonas fluorescens F113 genes implicated in flagellar filament synthesis and their role in competitive root colonization. Microbiology
150: 3889-3897
[Abstract] [Full Text] -
Urban, T. A., Griffith, A., Torok, A. M., Smolkin, M. E., Burns, J. L., Goldberg, J. B.
(2004). Contribution of Burkholderia cenocepacia Flagella to Infectivity and Inflammation. Infect. Immun.
72: 5126-5134
[Abstract] [Full Text] -
Shan, Z., Xu, H., Shi, X., Yu, Y., Yao, H., Zhang, X., Bai, Y., Gao, C., Saris, P. E. J., Qiao, M.
(2004). Identification of two new genes involved in twitching motility in Pseudomonas aeruginosa. Microbiology
150: 2653-2661
[Abstract] [Full Text] -
Spencer, D. H., Kas, A., Smith, E. E., Raymond, C. K., Sims, E. H., Hastings, M., Burns, J. L., Kaul, R., Olson, M. V.
(2003). Whole-Genome Sequence Variation among Multiple Isolates of Pseudomonas aeruginosa. J. Bacteriol.
185: 1316-1325
[Abstract] [Full Text] -
Scharfman, A., Arora, S. K., Delmotte, P., Van Brussel, E., Mazurier, J., Ramphal, R., Roussel, P.
(2001). Recognition of Lewis x Derivatives Present on Mucins by Flagellar Components of Pseudomonas aeruginosa. Infect. Immun.
69: 5243-5248
[Abstract] [Full Text] -
Arora, S. K., Bangera, M., Lory, S., Ramphal, R.
(2001). A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation. Proc. Natl. Acad. Sci. USA
98: 9342-9347
[Abstract] [Full Text] -
Tasteyre, A., Karjalainen, T., Avesani, V., Delmée, M., Collignon, A., Bourlioux, P., Barc, M.-C.
(2001). Molecular Characterization of fliD Gene Encoding Flagellar Cap and Its Expression among Clostridium difficile Isolates from Different Serogroups. J. Clin. Microbiol.
39: 1178-1183
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»