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Infection and Immunity, March 2000, p. 1700-1705, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytotoxic Cell Vacuolating Activity from Vibrio cholerae Hemolysin

Ana Coelho,1,* João R. C. Andrade,2 Ana Carolina P. Vicente,3 and Victor J. Dirita4

Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ21944-970,1 Serviço de Microbiologia e Immunologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ20551-030,2 and Departamento de Genética, Instituto Oswaldo Cruz, Rio de Janeiro, RJ21045-100,3 Brazil, and Unit for Laboratory Animal Medicine and Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 481094

Received 23 August 1999/Returned for modification 4 October 1999/Accepted 22 November 1999

A Vibrio cholerae cytotoxin, designated VcVac, was found to cause vacuolation in Vero cells. It was originally detected in the pathogenic O1 Amazonia variant of V. cholerae and later shown to be produced in environmental strains and some El Tor strains. Comparison of VcVac production in various strains suggested that hemolysin was responsible for the vacuolating phenotype. Genetic experiments established a firm correlation between vacuolation and hemolysin production. The mammalian cell vacuolating activity of the V. cholerae hemolysin is a new property of this protein and points to a previously unknown type of interaction between V. cholerae and its host.

* Corresponding author. Mailing address: Departamento de Genética, I. Biologia, UFRJ Cx Postal 68011, Ilha do Fundão, Rio de Janeiro RJ21944-970, Brazil. Phone and fax: (5521)260-2090. E-mail: coelho{at}biologia.ufrj.br.

Infection and Immunity, March 2000, p. 1700-1705, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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