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Infection and Immunity, March 2000, p. 1719-1723, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

T-Cell Epitopes in Variable Segments of Chlamydia trachomatis Major Outer Membrane Protein Elicit Serovar-Specific Immune Responses in Infected Humansdagger

Linette Ortiz,1,* Mark Angevine,2 Seon-Kyeong Kim,3 David Watkins,4 and Robert DeMars1,*

Laboratory of Genetics, University of Wisconsin,1 and Departments of Medicine,2 Medical Microbiology and Immunology,3 and Pathology,4 University of Wisconsin Medical School, Madison, Wisconsin 53706

Received 3 August 1999/Returned for modification 24 September 1999/Accepted 23 November 1999

We previously identified 18 stimulatory Chlamydia trachomatis major outer membrane protein (MOMP) peptides containing at least 23 epitopes presented with various HLA class II allotypes. Only one peptide contained an epitope localized in a variable segment (VS2). Continued studies reported here identified a total of five VS peptides containing T-cell epitopes that are distributed among MOMPs VS1, VS2, and VS4. Only MOMP-primed T-cell cultures from subjects infected with serovar E responded to the serovar E VS peptides, while the response of such cultures to constant-segment peptides was independent of the infecting serovar. Furthermore, MOMP-primed T cells proliferated in response only to the VS peptides encoded in serovar E but not to the corresponding peptides derived from serovar F, I, or J, confirming that these responses were serovar specific.

* Corresponding author. Present address for Linette Ortiz: Department of Pharmacology, Rm. 3635 MSC, University of Wisconsin, 1300 University Ave., Madison, WI 53706. Phone: (608) 265-0795. Fax: (608) 262-1257. E-mail: lortiz{at}facstaff.wisc.edu. Mailing address for Robert DeMars: Laboratory of Genetics, University of Wisconsin, 445 Henry Mall, Madison, WI 53706. Phone: (608) 262-3402. Fax: (608) 262-2976. E-mail: ridemars{at}facstaff.wisc.edu.

dagger This is paper 3491 from the Laboratory of Genetics, University of Wisconsin-Madison.

Infection and Immunity, March 2000, p. 1719-1723, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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