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Infection and Immunity, March 2000, p. 1740-1745, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Lipopolysaccharides of Brucella abortus and Brucella melitensis Induce Nitric Oxide Synthesis in Rat Peritoneal Macrophages

Luis López-Urrutia,1,2 Andrés Alonso,3 Maria Luisa Nieto,2 Yolanda Bayón,3 Antonio Orduña,1,2 and Mariano Sánchez Crespo3,*

Departamento de Microbiología, Facultad de Medicina,1 Unidad de Investigación, Hospital Clínico Universitario,2 and Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Cientificas,3 Valladolid, Spain

Received 28 October 1999/Returned for modification 16 November 1999/Accepted 6 December 1999

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS protein. These observations might help to explain (i) the acute outcome of Brucella infection in rodents, (ii) the low frequency of septic shock in human brucellosis, and (iii) the prolonged intracellular survival of Brucella in humans.

* Corresponding author. Mailing address: IBGM, Facultad de Medicina, 47005-Valladolid, Spain. Phone: 34-983-423273. Fax: 34-983-423588. E-mail: mscres{at}ibgm.uva.es.

Infection and Immunity, March 2000, p. 1740-1745, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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