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Infection and Immunity, April 2000, p. 1840-1848, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Enhanced Hematopoietic Activity Accompanies Parasite Expansion in the Spleen and Bone Marrow of Mice Infected with Leishmania donovani

Sara E. J. Cotterell, Christian R. Engwerda, and Paul M. Kaye*

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

Received 15 October 1999/Returned for modification 22 November 1999/Accepted 4 January 2000

In this study, we have analyzed hematopoietic activity in the spleen, bone marrow, and blood of BALB/c and scid mice infected with Leishmania donovani. Our analysis demonstrates that infection induces a rapid but transient mobilization of progenitor cells into the circulation, associated with elevated levels of granulocyte/macrophage colony-stimulating factor (GM-CSF) and MIP-1alpha . From 14 to 28 days postinfection, when parasite expansion begins in the spleen and bone marrow, both the frequency and cell cycle activity of hematopoietic progenitors, particulary CFU-granulocyte, monocyte, are dramatically increased in these organs. This is associated with increased accumulation of mRNA for GM-CSF, M-CSF, and G-CSF, but not interleukin-3. Our data also illustrate that hematopoietic activity, as assessed by changes in the frequency of progenitor cell populations and their levels of cell cycle activity, can be regulated in both a T-cell-independent and T-cell-dependent, as well as in an organ-specific, manner. Collectively, these data add to our knowledge of the long-term changes which occur in organs in which L. donovani is able to persist.

* Corresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT United Kingdom. Phone: 44 171 927 2390. Fax: 44 171 323 5687. E-mail: paul.kaye{at}lshtm.ac.uk.

Infection and Immunity, April 2000, p. 1840-1848, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • MacNamara, K. C., Racine, R., Chatterjee, M., Borjesson, D., Winslow, G. M. (2009). Diminished Hematopoietic Activity Associated with Alterations in Innate and Adaptive Immunity in a Mouse Model of Human Monocytic Ehrlichiosis. Infect. Immun. 77: 4061-4069 [Abstract] [Full Text]  
  • Engwerda, C. R., Ato, M., Cotterell, S. E. J., Mynott, T. L., Tschannerl, A., Gorak-Stolinska, P. M. A., Kaye, P. M. (2002). A Role for Tumor Necrosis Factor-{alpha} in Remodeling the Splenic Marginal Zone during Leishmania donovani Infection. Am. J. Pathol. 161: 429-437 [Abstract] [Full Text]  
  • Murray, H. W. (2001). Clinical and Experimental Advances in Treatment of Visceral Leishmaniasis. Antimicrob. Agents Chemother. 45: 2185-2197 [Full Text]  


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