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Infection and Immunity, April 2000, p. 1849-1854, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning, Sequencing, and Role in Serum Susceptibility of Porin II from Mesophilic Aeromonas hydrophila

Maria Mercé Nogueras,1 Susana Merino,1 Alicia Aguilar,1 Vicente Javier Benedi,2 and Juan M. Tomás1,*

Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, 08071 Barcelona,1 and Departamento de Biología Ambiental, Universidad de las Islas Baleares, Palma de Mallorca,2 Spain

Received 21 October 1999/Returned for modification 8 December 1999/Accepted 28 December 1999

We cloned and sequenced the structural gene for Aeromonas hydrophila porin II from strain AH-3 (serogroup O:34). The genetic position of this gene, like that of ompF in Escherichia coli, is adjacent to aspC and transcribed in the same direction. However, upstream of the porin II gene no similarities with E. coli were found. We obtained defined insertion mutants in porin II gene either in A. hydrophila (O:34) or A. veronii sobria (serogroup O:11) serum-resistant or -sensitive strains. Furthermore, we complemented these mutants with a plasmid harboring only the porin II gene, which allowed us to define the role of porin II as an important surface molecule involved in serum susceptibility and C1q binding in these strains.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 80871 Barcelona, Spain. Phone: 34-93-4021486. Fax: 34-93-4110592. E-mail: juant{at}bio.ub.es.

Infection and Immunity, April 2000, p. 1849-1854, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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