Previous Article | Next Article 
Infection and Immunity, April 2000, p. 1849-1854, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Cloning, Sequencing, and Role in Serum
Susceptibility of Porin II from Mesophilic Aeromonas
hydrophila
Maria Mercé
Nogueras,1
Susana
Merino,1
Alicia
Aguilar,1
Vicente Javier
Benedi,2 and
Juan M.
Tomás1,*
Departamento de Microbiología,
Facultad de Biología, Universidad de Barcelona, 08071 Barcelona,1 and Departamento de
Biología Ambiental, Universidad de las Islas Baleares, Palma de
Mallorca,2 Spain
Received 21 October 1999/Returned for modification 8 December
1999/Accepted 28 December 1999
We cloned and sequenced the structural gene for Aeromonas
hydrophila porin II from strain AH-3 (serogroup O:34). The
genetic position of this gene, like that of ompF in
Escherichia coli, is adjacent to aspC and
transcribed in the same direction. However, upstream of the porin II
gene no similarities with E. coli were found. We obtained
defined insertion mutants in porin II gene either in A. hydrophila (O:34) or A. veronii sobria (serogroup O:11) serum-resistant or -sensitive strains. Furthermore, we
complemented these mutants with a plasmid harboring only the porin II
gene, which allowed us to define the role of porin II as an important surface molecule involved in serum susceptibility and C1q binding in
these strains.
*
Corresponding author. Mailing address: Departamento de
Microbiología, Facultad de Biología, Universidad de
Barcelona, Diagonal 645, 80871 Barcelona, Spain. Phone: 34-93-4021486. Fax: 34-93-4110592. E-mail: juant{at}bio.ub.es.
Infection and Immunity, April 2000, p. 1849-1854, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Vilches, S., Jimenez, N., Tomas, J. M., Merino, S.
(2009). Aeromonas hydrophila AH-3 Type III Secretion System Expression and Regulatory Network. Appl. Environ. Microbiol.
75: 6382-6392
[Abstract]
[Full Text]
-
Jimenez, N., Canals, R., Salo, M. T., Vilches, S., Merino, S., Tomas, J. M.
(2008). The Aeromonas hydrophila wb*O34 Gene Cluster: Genetics and Temperature Regulation. J. Bacteriol.
190: 4198-4209
[Abstract]
[Full Text]
-
Jimenez, N., Canals, R., Lacasta, A., Kondakova, A. N., Lindner, B., Knirel, Y. A., Merino, S., Regue, M., Tomas, J. M.
(2008). Molecular Analysis of Three Aeromonas hydrophila AH-3 (Serotype O34) Lipopolysaccharide Core Biosynthesis Gene Clusters. J. Bacteriol.
190: 3176-3184
[Abstract]
[Full Text]
-
Vilches, S., Canals, R., Wilhelms, M., Salo, M. T., Knirel, Y. A., Vinogradov, E., Merino, S., Tomas, J. M.
(2007). Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulence. Microbiology
153: 2393-2404
[Abstract]
[Full Text]
-
Canals, R., Vilches, S., Wilhelms, M., Shaw, J. G., Merino, S., Tomas, J. M.
(2007). Non-structural flagella genes affecting both polar and lateral flagella-mediated motility in Aeromonas hydrophila. Microbiology
153: 1165-1175
[Abstract]
[Full Text]
-
Canals, R., Jimenez, N., Vilches, S., Regue, M., Merino, S., Tomas, J. M.
(2007). Role of Gne and GalE in the Virulence of Aeromonas hydrophila Serotype O34. J. Bacteriol.
189: 540-550
[Abstract]
[Full Text]
-
Canals, R., Altarriba, M., Vilches, S., Horsburgh, G., Shaw, J. G., Tomas, J. M., Merino, S.
(2006). Analysis of the Lateral Flagellar Gene System of Aeromonas hydrophila AH-3. J. Bacteriol.
188: 852-862
[Abstract]
[Full Text]
-
Canals, R., Ramirez, S., Vilches, S., Horsburgh, G., Shaw, J. G., Tomas, J. M., Merino, S.
(2006). Polar Flagellum Biogenesis in Aeromonas hydrophila. J. Bacteriol.
188: 542-555
[Abstract]
[Full Text]
-
Canals, R., Jimenez, N., Vilches, S., Regue, M., Merino, S., Tomas, J. M.
(2006). The UDP N-Acetylgalactosamine 4-Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence. Infect. Immun.
74: 537-548
[Abstract]
[Full Text]
-
Vilches, S., Urgell, C., Merino, S., Chacon, M. R., Soler, L., Castro-Escarpulli, G., Figueras, M. J., Tomas, J. M.
(2004). Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain. Appl. Environ. Microbiol.
70: 6914-6919
[Abstract]
[Full Text]
-
Braschler, T. R., Merino, S., Tomas, J. M., Graf, J.
(2003). Complement Resistance Is Essential for Colonization of the Digestive Tract of Hirudo medicinalis by Aeromonas Strains. Appl. Environ. Microbiol.
69: 4268-4271
[Abstract]
[Full Text]
-
Merino, S., Gavin, R., Vilches, S., Shaw, J. G., Tomas, J. M.
(2003). A Colonization Factor (Production of Lateral Flagella) of Mesophilic Aeromonas spp. Is Inactive in Aeromonas salmonicida Strains. Appl. Environ. Microbiol.
69: 663-667
[Abstract]
[Full Text]
-
Paquet, J.-Y., Diaz, M. A., Genevrois, S., Grayon, M., Verger, J.-M., De Bolle, X., Lakey, J. H., Letesson, J.-J., Cloeckaert, A.
(2001). Molecular, Antigenic, and Functional Analyses of Omp2b Porin Size Variants of Brucella spp.. J. Bacteriol.
183: 4839-4847
[Abstract]
[Full Text]