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Infection and Immunity, April 2000, p. 1879-1883, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Human Dendritic Cells Are Superior to B Cells at Presenting a Major Histocompatibility Complex Class II-Restricted Heterologous Antigen Expressed on Recombinant Streptococcus gordonii

Silvia Corinti,1,* Donata Medaglini,2 Caterina Prezzi,1 Andrea Cavani,1 Gianni Pozzi,2 and Giampiero Girolomoni1

Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, IRCCS, Rome,1 and Section of Microbiology, Department of Molecular Biology, University of Siena,2 Italy

Received 18 November 1999/Returned for modification 17 December 1999/Accepted 3 January 2000

Bacteria are being actively investigated as vaccine carriers for inducing or boosting protective immune responses. In this study, human monocyte-derived dendritic cells (DCs) and normal B cells were compared for their capacity to present the C fragment of tetanus toxin (TTFC), expressed on the surface of recombinant Streptococcus gordonii, to specific CD4+ T lymphocytes. DCs were more efficient than B cells at presenting soluble TTFC and remarkably more capable of presenting bacterium-associated TTFC both in terms of the amount of antigen required to obtain a given T-cell response and on a per-cell basis. This difference was associated with a much lower capacity of B cells to endocytose soluble TTFC and phagocytose recombinant S. gordonii. In addition, S. gordonii induced the phenotypic maturation of DCs but not of B cells. The results thus indicate that DCs but not B cells play a crucial role in the amplification of class II-restricted immune responses induced by immunization with recombinant gram-positive bacteria.

* Corresponding author. Mailing address: Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, IRCCS. Via dei Monti di Creta 104, 00167 Rome, Italy. Phone: 39-06-6646-4718. Fax: 39-06-6646-4705. E-mail: imm1{at}idi.it.

Infection and Immunity, April 2000, p. 1879-1883, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ciabattini, A., Pettini, E., Andersen, P., Pozzi, G., Medaglini, D. (2008). Primary Activation of Antigen-Specific Naive CD4+ and CD8+ T Cells following Intranasal Vaccination with Recombinant Bacteria. Infect. Immun. 76: 5817-5825 [Abstract] [Full Text]  
  • Ciabattini, A., Cuppone, A. M., Pulimeno, R., Iannelli, F., Pozzi, G., Medaglini, D. (2006). Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii.. CVI 13: 1037-1043 [Abstract] [Full Text]  
  • Medaglini, D., Ciabattini, A., Cuppone, A. M., Costa, C., Ricci, S., Costalonga, M., Pozzi, G. (2006). In Vivo Activation of Naive CD4+ T Cells in Nasal Mucosa-Associated Lymphoid Tissue following Intranasal Immunization with Recombinant Streptococcus gordonii.. Infect. Immun. 74: 2760-2766 [Abstract] [Full Text]  


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