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Infection and Immunity, April 2000, p. 1934-1941, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Adhesins and Toxins in Invasion of Human Tracheal Epithelial Cells by Bordetella pertussis

Laurence Bassinet,1,2 Pascale Gueirard,1 Bernard Maitre,2 Bruno Housset,2 Pierre Gounon,3 and Nicole Guiso1,*

Centre Hospitalier Intercommunal de Créteil, Service de Pneumologie, Créteil,2 and Laboratoire des Bordetella,1 and Station Centrale de Microscopie Electronique,3 Institut Pasteur, Paris, France

Received 3 September 1999/Returned for modification 15 December 1999/Accepted 19 January 2000

Bordetella pertussis, the agent of whooping cough, can invade and survive in several types of eukaryotic cell, including CHO, HeLa 229, and HEp-2 cells and macrophages. In this study, we analyzed bacterial invasiveness in nonrespiratory human HeLa epithelial cells and human HTE and HAE0 tracheal epithelial cells. Invasion assays and transmission electron microscopy analysis showed that B. pertussis strains invaded and survived, without multiplying, in HTE or HAE0 cells. This phenomenon was bvg regulated, but invasive properties differed between B. pertussis strains and isolates and the B. pertussis reference strain. Studies with B. pertussis mutant strains demonstrated that filamentous hemagglutinin, the major adhesin, was involved in the invasion of human tracheal epithelial cells by bacteria but not in that of HeLa cells. Fimbriae and pertussis toxin were not found to be involved. However, we found that the production of adenylate cyclase-hemolysin prevents the invasion of HeLa and HTE cells by B. pertussis because an adenylate cyclase-hemolysin-deficient mutant was found to be more invasive than the parental strain. The effect of adenylate cyclase-hemolysin was mediated by an increase in the cyclic AMP concentration in the cells. Pertactin (PRN), an adhesin, significantly inhibited the invasion of HTE cells by bacteria, probably via its interaction with adenylate cyclase-hemolysin. Isolates producing different PRNs were taken up similarly, indicating that the differences in the sequences of the PRNs produced by these isolates do not affect invasion. We concluded that filamentous hemagglutinin production favored invasion of human tracheal cells but that adenylate cyclase-hemolysin and PRN production significantly inhibited this process.

* Corresponding author. Mailing address: Laboratoire des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33-1) 45.68.83.34. Fax: (33-1) 40.61.35.33. E-mail: nguiso{at}pasteur.fr.

Infection and Immunity, April 2000, p. 1934-1941, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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J. Bacteriol. J. Virol. Eukaryot. Cell
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Copyright © 2000 by the American Society for Microbiology. All rights reserved.