This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Geng, Y.
Right arrow Articles by Trinchieri, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geng, Y.
Right arrow Articles by Trinchieri, G.

 Previous Article  |  Next Article 

Infection and Immunity, April 2000, p. 2245-2253, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Roles of Interleukin-12 and Gamma Interferon in Murine Chlamydia pneumoniae Infection

Yuemei Geng,1,dagger Klara Berencsi,1 Zsofia Gyulai,1 Tibor Valyi-Nagy,2 Eva Gonczol,1 and Giorgio Trinchieri1,*

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104,1 and Vanderbilt University Medical Center, Nashville, Tennessee 372322

Received 22 July 1999/Returned for modification 21 October 1999/Accepted 2 December 1999

BALB/c and strain 129 mice infected intranasally with Chlamydia pneumoniae displayed a moderate-to-severe inflammation in the lungs and produced interleukin-12 (IL-12), gamma interferon (IFN-gamma ), tumor necrosis factor alpha (TNF-alpha ), and IL-10, with peak levels on days 1 to 3 postinfection (p.i.), returning to basal levels by day 16 p.i. Anti-IL-12 treatment resulted in less-severe pathological changes but higher bacterial titers on days 3 and 7 p.i. By day 16 p.i., the inflammatory responses of control antibody-treated mice subsided. The bacterial titers of both anti-IL-12- and control antibody-treated mice decreased within 3 weeks to marginally detectable levels. Anti-IL-12 treatment significantly reduced lung IFN-gamma production and in vitro spleen cell IFN-gamma production in response to either C. pneumoniae or concanavalin A. In gamma -irradiated infected mice, cytokine production was delayed, and this delay correlated with high bacterial titers in the lungs. Following C. pneumoniae infection, 129 mice lacking the IFN-gamma receptor alpha  chain gene (G129 mice) produced similar IL-12 levels and exhibited similarly severe pathological changes but had higher bacterial titers than 129 mice. However, by day 45 p.i., bacterial titers became undetectable in both wild-type 129 and G129 mice. Thus, during C. pneumoniae lung infection, IL-12, more than IFN-gamma , plays a role in pulmonary-cell infiltration. IFN-gamma and IL-12, acting mostly through its induction of IFN-gamma and Th1 responses, play an important role in controlling acute C. pneumoniae infection in the lungs, but eventually all mice control the infection to undetectable levels by IL-12- and IFN-gamma -independent mechanisms.

* Corresponding author. Present address: Schering-Plough Laboratory of Immunological Research, 27 Chemin des Peupliers, BP 11, 69571 Dardilly Cedex, France. Phone: 33 472 17 2740. Fax: 33 478 35 4750. E-mail: giorgio.trinchieri{at}spcorp.com.

dagger Present address: Department of Dermatology, University of Pennsylvania, Philadelphia, PA 19104.

Infection and Immunity, April 2000, p. 2245-2253, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhou, X., Chen, Q., Moore, J., Kolls, J. K., Halperin, S., Wang, J. (2009). Critical Role of the Interleukin-17/Interleukin-17 Receptor Axis in Regulating Host Susceptibility to Respiratory Infection with Chlamydia Species. Infect. Immun. 77: 5059-5070 [Abstract] [Full Text]  
  • Bai, H., Cheng, J., Gao, X., Joyee, A. G., Fan, Y., Wang, S., Jiao, L., Yao, Z., Yang, X. (2009). IL-17/Th17 Promotes Type 1 T Cell Immunity against Pulmonary Intracellular Bacterial Infection through Modulating Dendritic Cell Function. J. Immunol. 183: 5886-5895 [Abstract] [Full Text]  
  • Rodriguez, N., Lang, R., Wantia, N., Cirl, C., Ertl, T., Durr, S., Wagner, H., Miethke, T. (2008). Induction of iNOS by Chlamydophila pneumoniae requires MyD88-dependent activation of JNK. J. Leukoc. Biol. 84: 1585-1593 [Abstract] [Full Text]  
  • De Pascalis, R., Taylor, B. C., Elkins, K. L. (2008). Diverse Myeloid and Lymphoid Cell Subpopulations Produce Gamma Interferon during Early Innate Immune Responses to Francisella tularensis Live Vaccine Strain. Infect. Immun. 76: 4311-4321 [Abstract] [Full Text]  
  • Ruan, S., McKinley, L., Zheng, M., Rudner, X., D'Souza, A., Kolls, J. K., Shellito, J. E. (2008). Interleukin-12 and Host Defense against Murine Pneumocystis Pneumonia. Infect. Immun. 76: 2130-2137 [Abstract] [Full Text]  
  • Horvat, J. C., Beagley, K. W., Wade, M. A., Preston, J. A., Hansbro, N. G., Hickey, D. K., Kaiko, G. E., Gibson, P. G., Foster, P. S., Hansbro, P. M. (2007). Neonatal Chlamydial Infection Induces Mixed T-Cell Responses That Drive Allergic Airway Disease. Am. J. Respir. Crit. Care Med. 176: 556-564 [Abstract] [Full Text]  
  • Eickhoff, M., Thalmann, J., Hess, S., Martin, M., Laue, T., Kruppa, J., Brandes, G., Klos, A. (2007). Host Cell Responses to Chlamydia pneumoniae in Gamma Interferon-Induced Persistence Overlap Those of Productive Infection and Are Linked to Genes Involved in Apoptosis, Cell Cycle, and Metabolism. Infect. Immun. 75: 2853-2863 [Abstract] [Full Text]  
  • Joyee, A. G., Qiu, H., Wang, S., Fan, Y., Bilenki, L., Yang, X. (2007). Distinct NKT Cell Subsets Are Induced by Different Chlamydia Species Leading to Differential Adaptive Immunity and Host Resistance to the Infections. J. Immunol. 178: 1048-1058 [Abstract] [Full Text]  
  • Happel, K. I., Dubin, P. J., Zheng, M., Ghilardi, N., Lockhart, C., Quinton, L. J., Odden, A. R., Shellito, J. E., Bagby, G. J., Nelson, S., Kolls, J. K. (2005). Divergent roles of IL-23 and IL-12 in host defense against Klebsiella pneumoniae. JEM 202: 761-769 [Abstract] [Full Text]  
  • Rodriguez, N., Fend, F., Jennen, L., Schiemann, M., Wantia, N., da Costa, C. U. P., Durr, S., Heinzmann, U., Wagner, H., Miethke, T. (2005). Polymorphonuclear Neutrophils Improve Replication of Chlamydia pneumoniae In Vivo upon MyD88-Dependent Attraction. J. Immunol. 174: 4836-4844 [Abstract] [Full Text]  
  • Fonseca-Aten, M., Rios, A. M., Mejias, A., Chavez-Bueno, S., Katz, K., Gomez, A. M., McCracken, G. H. Jr., Hardy, R. D. (2005). Mycoplasma pneumoniae Induces Host-Dependent Pulmonary Inflammation and Airway Obstruction in Mice. Am. J. Respir. Cell Mol. Bio. 32: 201-210 [Abstract] [Full Text]  
  • de Kruif, M. D., van Gorp, E. C.M., Keller, T. T., Ossewaarde, J. M., ten Cate, H. (2005). Chlamydia pneumoniae infections in mouse models: relevance for atherosclerosis research. Cardiovasc Res 65: 317-327 [Abstract] [Full Text]  
  • Netea, M. G., Kullberg, B. J., Jacobs, L. E. H., Verver-Jansen, T. J. G., van der Ven-Jongekrijg, J., Galama, J. M. D., Stalenhoef, A. F. H., Dinarello, C. A., Van der Meer, J. W. M. (2004). Chlamydia pneumoniae Stimulates IFN-{gamma} Synthesis through MyD88-Dependent, TLR2- and TLR4-Independent Induction of IL-18 Release. J. Immunol. 173: 1477-1482 [Abstract] [Full Text]  
  • Yang, J., Hooper, W. C., Phillips, D. J., Tondella, M. L., Talkington, D. F. (2003). Induction of Proinflammatory Cytokines in Human Lung Epithelial Cells during Chlamydia pneumoniae Infection. Infect. Immun. 71: 614-620 [Abstract] [Full Text]  
  • Saren, A., Pascolo, S., Stevanovic, S., Dumrese, T., Puolakkainen, M., Sarvas, M., Rammensee, H.-G., Vuola, J. M. (2002). Identification of Chlamydia pneumoniae-Derived Mouse CD8 Epitopes. Infect. Immun. 70: 3336-3343 [Abstract] [Full Text]  
  • Kutlin, A., Roblin, P. M., Hammerschlag, M. R. (2002). Effect of gemifloxacin on viability of Chlamydia pneumoniae (Chlamydophila pneumoniae) in an in vitro continuous infection model. J Antimicrob Chemother 49: 763-767 [Abstract] [Full Text]  
  • Elkins, K. L., Cooper, A., Colombini, S. M., Cowley, S. C., Kieffer, T. L. (2002). In Vivo Clearance of an Intracellular Bacterium, Francisella tularensis LVS, Is Dependent on the p40 Subunit of Interleukin-12 (IL-12) but Not on IL-12 p70. Infect. Immun. 70: 1936-1948 [Abstract] [Full Text]  
  • Haranaga, S., Yamaguchi, H., Friedman, H., Izumi, S.-i., Yamamoto, Y. (2001). Chlamydia pneumoniae Infects and Multiplies in Lymphocytes In Vitro. Infect. Immun. 69: 7753-7759 [Abstract] [Full Text]  
  • Gigliotti Rothfuchs, A., Gigliotti, D., Palmblad, K., Andersson, U., Wigzell, H., Rottenberg, M. E. (2001). IFN-{alpha}{beta}-Dependent, IFN-{gamma} Secretion by Bone Marrow-Derived Macrophages Controls an Intracellular Bacterial Infection. J. Immunol. 167: 6453-6461 [Abstract] [Full Text]  
  • Burian, K., Berencsi, K., Endresz, V., Gyulai, Z., Valyi-Nagy, T., Valyi-Nagy, I., Bakay, M., Geng, Y., Virok, D., Kari, L., Hajnal-Papp, R., Trinchieri, G., Gonczol, E. (2001). Chlamydia pneumoniae Exacerbates Aortic Inflammatory Foci Caused by Murine Cytomegalovirus Infection in Normocholesterolemic Mice. CVI 8: 1263-1266 [Abstract] [Full Text]  
  • Del Rio, L., Buendia, A. J., Sanchez, J., Gallego, M. C., Caro, M. R., Ortega, N., Seva, J., Pallares, F. J., Cuello, F., Salinas, J. (2001). Endogenous Interleukin-12 Is Not Required for Resolution of Chlamydophila abortus (Chlamydia psittaci Serotype 1) Infection in Mice. Infect. Immun. 69: 4808-4815 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»