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Infection and Immunity, April 2000, p. 2323-2327, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Identification of Secreted Proteins of
Mycobacterium tuberculosis by a Bioinformatic
Approach
Manuel
Gomez,
Sadie
Johnson, and
Maria Laura
Gennaro*
Public Health Research Institute, New York,
New York
Received 3 September 1999/Returned for modification 18 October
1999/Accepted 13 December 1999
Proteins secreted by Mycobacterium tuberculosis are
usually targets of immune responses in the infected host. Here we
describe a search for secreted proteins that combined the use of
bioinformatics and phoA' fusion technology. The 3,924 proteins deduced from the M. tuberculosis genome were
analyzed with several computer programs. We identified 52 proteins
carrying an NH2-terminal secretory signal peptide but
lacking additional membrane-anchoring moieties. Of these 52 proteins
the TM1 subgroup
only 7 had been previously reported to be
secreted proteins. Our predictions were confirmed in 9 of 10 TM1 genes
that were fused to Escherichia coli phoA', a marker of
subcellular localization. These findings demonstrate that the
systematic computer search described in this work identified secreted
proteins of M. tuberculosis with high efficiency and 90% accuracy.
*
Corresponding author. Mailing address: Public Health
Research Institute, 455 First Ave., New York, NY 10016. Phone: (212) 578-0844. Fax: (212) 578-0804. E-mail:
gennaro{at}phri.nyu.edu.
Infection and Immunity, April 2000, p. 2323-2327, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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