This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liang, F. T.
Right arrow Articles by Philipp, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liang, F. T.
Right arrow Articles by Philipp, M. T.

 Previous Article  |  Next Article 

Infection and Immunity, April 2000, p. 2349-2352, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Epitope Mapping of the Immunodominant Invariable Region of Borrelia burgdorferi VlsE in Three Host Species

Fang Ting Liang and Mario T. Philipp*

Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433

Received 3 November 1999/Returned for modification 1 December 1999/Accepted 21 December 1999

VlsE, the variable surface antigen of Borrelia burgdorferi, contains a 26-amino-acid-long immunodominant invariable region, IR6. In the present study, three overlapping 14-mer peptides reproducing the sequence of IR6 were used as peptide-based enzyme-linked immunosorbent assay antigens to map this invariable region in infected monkeys, mice, and human Lyme disease patients. Antibodies of the two primate species appeared to recognize IR6 as a single antigenic determinant, while mouse antibodies recognized multiple epitopes within this region.

* Corresponding author. Mailing address: Tulane Regional Primate Research Center, Tulane University Medical Center, 18703 Three Rivers Rd., Covington, LA 70433. Phone: (504) 871-6221. Fax: (504) 871-6390. E-mail: philipp{at}tpc.tulane.edu.

Infection and Immunity, April 2000, p. 2349-2352, Vol. 68, No. 4
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gomes-Solecki, M. J. C., Meirelles, L., Glass, J., Dattwyler, R. J. (2007). Epitope Length, Genospecies Dependency, and Serum Panel Effect in the IR6 Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Borrelia burgdorferi. CVI 14: 875-879 [Abstract] [Full Text]  
  • O'Connor, T. P., Esty, K. J., Hanscom, J. L., Shields, P., Philipp, M. T. (2004). Dogs Vaccinated with Common Lyme Disease Vaccines Do Not Respond to IR6, the Conserved Immunodominant Region of the VlsE Surface Protein of Borrelia burgdorferi. CVI 11: 458-462 [Abstract] [Full Text]  
  • Eicken, C., Sharma, V., Klabunde, T., Lawrenz, M. B., Hardham, J. M., Norris, S. J., Sacchettini, J. C. (2002). Crystal Structure of Lyme Disease Variable Surface Antigen VlsE of Borrelia burgdorferi. J. Biol. Chem. 277: 21691-21696 [Abstract] [Full Text]  
  • Liang, F. T., Bowers, L. C., Philipp, M. T. (2001). C-Terminal Invariable Domain of VlsE Is Immunodominant but Its Antigenicity Is Scarcely Conserved among Strains of Lyme Disease Spirochetes. Infect. Immun. 69: 3224-3231 [Abstract] [Full Text]  
  • Liang, F. T., Jacobson, R. H., Straubinger, R. K., Grooters, A., Philipp, M. T. (2000). Characterization of a Borrelia burgdorferi VlsE Invariable Region Useful in Canine Lyme Disease Serodiagnosis by Enzyme-Linked Immunosorbent Assay. J. Clin. Microbiol. 38: 4160-4166 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»