This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujiwara, T.
Right arrow Articles by Hamada, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujiwara, T.
Right arrow Articles by Hamada, S.

 Previous Article  |  Next Article 

Infection and Immunity, May 2000, p. 2475-2483, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Purification, Characterization, and Molecular Analysis of the Gene Encoding Glucosyltransferase from Streptococcus oralis

Taku Fujiwara,1,* Tomonori Hoshino,1 Takashi Ooshima,1 Shizuo Sobue,1 and Shigeyuki Hamada2

Departments of Pedodontics1 and Oral Microbiology,2 Osaka University Faculty of Dentistry, Suita-Osaka 565-0871, Japan

Received 22 November 1999/Returned for modification 21 December 1999/Accepted 19 January 2000

Streptococcus oralis is a member of the oral streptococcal family and an early-colonizing microorganism in the oral cavity of humans. S. oralis is known to produce glucosyltransferase (GTase), which synthesizes glucans from sucrose. The enzyme was purified chromatographically from a culture supernatant of S. oralis ATCC 10557. The purified enzyme, GTase-R, had a molecular mass of 173 kDa and a pI of 6.3. This enzyme mainly synthesized water-soluble glucans with no primer dependency. The addition of GTase markedly enhanced the sucrose-dependent resting cell adhesion of Streptococcus mutans at a level similar to that found in growing cells of S. mutans. The antibody against GTase-R inhibited the glucan-synthesizing activities of Streptococcus gordonii and Streptococcus sanguis, as well as S. oralis. The N-terminal amino acid sequence of GTase-R exhibited no similarities to known GTase sequences of oral streptococci. Using degenerate PCR primers, an 8.1-kb DNA fragment, carrying the gene (gtfR) coding for GTase-R and its regulator gene (rgg), was cloned and sequenced. Comparison of the deduced amino acid sequence revealed that the rgg genes of S. oralis and S. gordonii exhibited a close similarity. The gtfR gene was found to possess a species-specific nucleotide sequence corresponding to the N-terminal 130 amino acid residues. Insertion of erm or aphA into the rgg or gtfR gene resulted in decreased GTase activity by the organism and changed the colony morphology of these transformants. These results indicate that S. oralis GTase may play an important role in the subsequent colonizing of mutans streptoccoci.

* Corresponding author. Mailing address: Department of Pedodontics, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita-Osaka, 565-0871, Japan. Phone: 81-6-6879-2962. Fax: 81-6-6879-2965. E-mail: fujiwara{at}dent.osaka-u.ac.jp.

Infection and Immunity, May 2000, p. 2475-2483, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nakano, K., Lapirattanakul, J., Nomura, R., Nemoto, H., Alaluusua, S., Gronroos, L., Vaara, M., Hamada, S., Ooshima, T., Nakagawa, I. (2007). Streptococcus mutans Clonal Variation Revealed by Multilocus Sequence Typing. J. Clin. Microbiol. 45: 2616-2625 [Abstract] [Full Text]  
  • Xu, P., Alves, J. M., Kitten, T., Brown, A., Chen, Z., Ozaki, L. S., Manque, P., Ge, X., Serrano, M. G., Puiu, D., Hendricks, S., Wang, Y., Chaplin, M. D., Akan, D., Paik, S., Peterson, D. L., Macrina, F. L., Buck, G. A. (2007). Genome of the Opportunistic Pathogen Streptococcus sanguinis. J. Bacteriol. 189: 3166-3175 [Abstract] [Full Text]  
  • Loughman, J. A., Caparon, M. G. (2007). Contribution of Invariant Residues to the Function of Rgg Family Transcription Regulators. J. Bacteriol. 189: 650-655 [Abstract] [Full Text]  
  • Dmitriev, A. V., McDowell, E. J., Kappeler, K. V., Chaussee, M. A., Rieck, L. D., Chaussee, M. S. (2006). The Rgg Regulator of Streptococcus pyogenes Influences Utilization of Nonglucose Carbohydrates, Prophage Induction, and Expression of the NAD-Glycohydrolase Virulence Operon.. J. Bacteriol. 188: 7230-7241 [Abstract] [Full Text]  
  • Rawlinson, E. L. A., Nes, I. F., Skaugen, M. (2005). Identification of the DNA-binding site of the Rgg-like regulator LasX within the lactocin S promoter region. Microbiology 151: 813-823 [Abstract] [Full Text]  
  • Tamesada, M., Kawabata, S., Fujiwara, T., Hamada, S. (2004). Synergistic Effects of Streptococcal Glucosyltransferases on Adhesive Biofilm Formation. JDR 83: 874-879 [Abstract] [Full Text]  
  • Neely, M. N., Lyon, W. R., Runft, D. L., Caparon, M. (2003). Role of RopB in Growth Phase Expression of the SpeB Cysteine Protease of Streptococcus pyogenes. J. Bacteriol. 185: 5166-5174 [Abstract] [Full Text]  
  • Banas, J.A., Vickerman, M.M. (2003). GLUCAN-BINDING PROTEINS OF THE ORAL STREPTOCOCCI. CROBM 14: 89-99 [Abstract] [Full Text]  
  • Ajdic', D., McShan, W. M., McLaughlin, R. E., Savic', G., Chang, J., Carson, M. B., Primeaux, C., Tian, R., Kenton, S., Jia, H., Lin, S., Qian, Y., Li, S., Zhu, H., Najar, F., Lai, H., White, J., Roe, B. A., Ferretti, J. J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99: 14434-14439 [Abstract] [Full Text]  
  • Nakano, K., Matsumura, M., Kawaguchi, M., Fujiwara, T., Sobue, S., Nakagawa, I., Hamada, S., Ooshima, T. (2002). Attenuation of Glucan-binding Protein C Reduces the Cariogenicity of Streptococcus mutans: Analysis of Strains Isolated from Human Blood. JDR 81: 376-379 [Abstract] [Full Text]  
  • Vickerman, M. M., Minick, P. E. (2002). Genetic Analysis of the rgg-gtfG Junctional Region and Its Role in Streptococcus gordonii Glucosyltransferase Activity. Infect. Immun. 70: 1703-1714 [Abstract] [Full Text]  
  • Skaugen, M., Andersen, E. L., Christie, V. H., Nes, I. F. (2002). Identification, Characterization, and Expression of a Second, Bicistronic, Operon Involved in the Production of Lactocin S in Lactobacillus sakei L45. Appl. Environ. Microbiol. 68: 720-727 [Abstract] [Full Text]  
  • Fujiwara, T., Hoshino, T., Ooshima, T., Hamada, S. (2002). Differential and Quantitative Analyses of mRNA Expression of Glucosyltransferases from Streptococcus mutans MT8148. JDR 81: 109-113 [Abstract] [Full Text]  
  • Vickerman, M. M., Minick, P. E., Mather, N. M. (2001). Characterization of the Streptococcus gordonii chromosomal region immediately downstream of the glucosyltransferase gene. Microbiology 147: 3061-3070 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»