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Infection and Immunity, May 2000, p. 2566-2572, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Outer Membrane Protein A, Peptidoglycan-Associated Lipoprotein, and Murein Lipoprotein Are Released by Escherichia coli Bacteria into Serum

Judith Hellman,1,* Paul M. Loiselle,2 Megan M. Tehan,1 Jennifer E. Allaire,2 Lenora A. Boyle,3 James T. Kurnick,3 David M. Andrews,3 Kwang Sik Kim,4 and H. Shaw Warren1,5

Departments of Anesthesia and Critical Care,2 Infectious Diseases,1 Pathology,3 and Pediatrics,5 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, and Department of Pediatrics, Childrens Hospital of Los Angeles and University of Southern California School of Medicine, Los Angeles, California4

Complexes containing lipopolysaccharide (LPS) and three outer membrane proteins (OMPs) are released by gram-negative bacteria incubated in human serum and into the circulation in an experimental model of sepsis. The same OMPs are bound by immunoglobulin G (IgG) in the cross-protective antiserum raised to Escherichia coli J5 (anti-J5 IgG). This study was performed to identify the three OMPs. The 35-kDa OMP was identified as outer membrane protein A (OmpA) by immunoblotting studies using OmpA-deficient bacteria and recombinant OmpA protein. The 18-kDa OMP was identified as peptidoglycan-associated lipoprotein (PAL) based on peptide sequences from the purified protein and immunoblotting studies using PAL-deficient bacteria. The 5- to 9-kDa OMP was identified as murein lipoprotein (MLP) based on immunoblotting studies using MLP-deficient bacteria. The studies identify the OMPs released into human serum and into the circulation in an experimental model of sepsis as OmpA, PAL, and MLP.

* Corresponding author. Mailing address: Infectious Disease Unit, 5th Floor, 149 Thirteenth St., Charlestown, MA 02129. Phone: (617) 724-3104 or 724-4959. Fax: (617) 726-4176. E-mail: hellman{at}etherdome.mgh.harvard.edu.

Infection and Immunity, May 2000, p. 2566-2572, Vol. 68, No. 5
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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