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Infection and Immunity, May 2000, p. 2573-2578, Vol. 68, No. 5
0019-9567/00/$04.00+0

Antigen Specificity in Experimental Bovine Tuberculosis

S. G. Rhodes,1,* D. Gavier-Widen,1,dagger B. M. Buddle,2 A. O. Whelan,1 M. Singh,3 R. G. Hewinson,1 and H. M. Vordermeier1

TB Research Group, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, United Kingdom1; AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand2; and GBF, D38124 Braunschweig, Germany3

Received 12 October 1999/Returned for modification 15 February 2000/Accepted 22 February 2000

This report describes the kinetics of T-cell responses to a panel of mycobacterial antigens (PPD-M, PPD-A, ESAT-6, Ag85, 38kD, MPB64, MPB70, MPB83, hsp16.1, hsp65, and hsp70) following experimental infection of cattle with Mycobacterium bovis. Increased antigen-specific lymphocyte proliferation, gamma interferon, and interleukin-2 responses were observed in all calves following infection. Positive lymphocyte proliferation and cytokine responses to PPD-M and ESAT-6 were observed throughout the infection period studied. In contrast, responses to all other antigens were more variable and were not constantly present, suggesting that antigen cocktails rather than individual antigens should be used for immunodiagnosis. The detection of cytokine responses in the absence of lymphocyte proliferation, particularly during the early stages of infection, suggests a role for antigen-specific cytokine readout systems in the early identification of M. bovis infection in cattle.

* Corresponding author. Mailing address: VLA, Addlestone, Surrey KT15 3NB, United Kingdom. Phone: 01932-341111. Fax: 01932-357684. E-mail: srhodes.vla{at}gtnet.gov.uk.

dagger Present address: Division of Wildlife, SVA, Uppsala, Sweden.

Infection and Immunity, May 2000, p. 2573-2578, Vol. 68, No. 5
0019-9567/00/$04.00+0


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